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• W2014641657 abstract "A very simple and efficient method was developed for isolating intact White Spot Syndrome Virus (WSSV) particles from infected Litopenaeus vannamei tissue. No density gradient centrifugation, ultracentrifugation or protease inhibitors were required for the purification of intact WSSV virions using microfilters (100 kDa cut-off) combined with several steps of conventional centrifugation procedures. A mortality assay was run using healthy shrimp to prove that the virions obtained were infective. The concentrated viral preparations were further studied using polyacrylamide gel electrophoresis (PAGE). At least five distinct protein bands were detected when intact purified WSSV virions were found by sodium dodecyl sulphate-PAGE, followed by Coomassie Brilliant R-250 staining. The estimated molecular weights of these proteins were 23, 24, 29, 32 and 42-kDa, which could correspond to viral protein. Using this method, the virus does not lose its ability to infect healthy shrimp." @default.
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• W2014641657 date "2009-04-01" @default.
• W2014641657 modified "2023-09-27" @default.
• W2014641657 title "A simple method for purifying the White Spot Syndrome Virus using ultrafiltration" @default.
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• W2014641657 doi "https://doi.org/10.1111/j.1365-2109.2008.02155.x" @default.
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