Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014649079> ?p ?o ?g. }
- W2014649079 endingPage "470" @default.
- W2014649079 startingPage "461" @default.
- W2014649079 abstract "Melanocarpus albomyces, a thermophilic fungus isolated from compost by enrichment culture in a liquid medium containing sugarcane bagasse, produced cellulase-free xylanase in culture medium. The fungus was unusual in that xylanase activity was inducible not only by hemicellulosic material but also by the monomeric pentosan unit of xylan but not by glucose. Concentration of bagasse-grown culture filtrate protein followed by size-exclusion and anion-exchange chromatography separated four xylanase activities. Under identical conditions of protein purification, xylanase I was absent in the xylose-grown culture filtrate. Two xylanase activities, a minor xylanase IA and a major xylanase IIIA, were purified to apparent homogeneity from bagasse-grown cultures. Both xylanases were specific forβ-1,4 xylose-rich polymer, optimally active, respectively, at pH 6.6 and 5.6, and at 65°C. The xylanases were stable between pH 5 to 10 at 50°C for 24 h. Xylanases released xylobiose, xylotriose and higher oligomers from xylans from different sources. Xylanase IA had a Mr of 38 kDa and contained 7% carbohydrate whereas xylanase IIIA had a Mr of 24 kDa and no detectable carbohydrate. The Km for larchwood xylan (mg ml−1) and Vmax (μmol xylose min−1 mg−1 protein) of xylanase IA were 0.33 and 311, and of xylanase IIIA 1.69 and 500, respectively. Xylanases IA, II and IIIA showed no synergism in the hydrolysis of larchwood glucuronoxylan or oat spelt and sugarcane bagasse arabinoxylans. They had different reactivity on untreated and delignified bagasse. The xylanases were more reactive than cellulase on delignified bagasse. Simultaneous treatment of delignified bagasse by xylanase and cellulase released more sugar than individual enzyme treatments. By contrast, the primary cell walls of a plant, particularly from the region of elongation, were more susceptible to the action of cellulase than xylanase. The effects of xylanase and cellulase on plant cell walls were consistent with the view that hemicellulose surrounds cellulose in plant cell walls." @default.
- W2014649079 created "2016-06-24" @default.
- W2014649079 creator A5044312898 @default.
- W2014649079 creator A5050924057 @default.
- W2014649079 date "1999-12-01" @default.
- W2014649079 modified "2023-10-14" @default.
- W2014649079 title "Biochemical properties of xylanases from a thermophilic fungus,Melanocarpus albomyces, and their action on plant cell walls" @default.
- W2014649079 cites W1775749144 @default.
- W2014649079 cites W1964568732 @default.
- W2014649079 cites W1964680581 @default.
- W2014649079 cites W1968834637 @default.
- W2014649079 cites W1973996850 @default.
- W2014649079 cites W2006462351 @default.
- W2014649079 cites W2012277065 @default.
- W2014649079 cites W2023071900 @default.
- W2014649079 cites W2038311099 @default.
- W2014649079 cites W2043624786 @default.
- W2014649079 cites W2043940975 @default.
- W2014649079 cites W2045309702 @default.
- W2014649079 cites W2045781385 @default.
- W2014649079 cites W2055349148 @default.
- W2014649079 cites W2056357096 @default.
- W2014649079 cites W2067819639 @default.
- W2014649079 cites W2094330771 @default.
- W2014649079 cites W2094864991 @default.
- W2014649079 cites W2106518453 @default.
- W2014649079 cites W2110624281 @default.
- W2014649079 cites W2115658273 @default.
- W2014649079 cites W2322368795 @default.
- W2014649079 cites W241276834 @default.
- W2014649079 cites W4231121696 @default.
- W2014649079 cites W4244554171 @default.
- W2014649079 cites W4247237823 @default.
- W2014649079 doi "https://doi.org/10.1007/bf02942657" @default.
- W2014649079 hasPublicationYear "1999" @default.
- W2014649079 type Work @default.
- W2014649079 sameAs 2014649079 @default.
- W2014649079 citedByCount "43" @default.
- W2014649079 countsByYear W20146490792012 @default.
- W2014649079 countsByYear W20146490792013 @default.
- W2014649079 countsByYear W20146490792014 @default.
- W2014649079 countsByYear W20146490792015 @default.
- W2014649079 countsByYear W20146490792016 @default.
- W2014649079 countsByYear W20146490792019 @default.
- W2014649079 countsByYear W20146490792020 @default.
- W2014649079 countsByYear W20146490792021 @default.
- W2014649079 countsByYear W20146490792022 @default.
- W2014649079 countsByYear W20146490792023 @default.
- W2014649079 crossrefType "journal-article" @default.
- W2014649079 hasAuthorship W2014649079A5044312898 @default.
- W2014649079 hasAuthorship W2014649079A5050924057 @default.
- W2014649079 hasBestOaLocation W20146490792 @default.
- W2014649079 hasConcept C100544194 @default.
- W2014649079 hasConcept C116174643 @default.
- W2014649079 hasConcept C134643618 @default.
- W2014649079 hasConcept C141603559 @default.
- W2014649079 hasConcept C150903083 @default.
- W2014649079 hasConcept C181199279 @default.
- W2014649079 hasConcept C185592680 @default.
- W2014649079 hasConcept C2775859485 @default.
- W2014649079 hasConcept C2779920640 @default.
- W2014649079 hasConcept C2780294150 @default.
- W2014649079 hasConcept C27911776 @default.
- W2014649079 hasConcept C31903555 @default.
- W2014649079 hasConcept C55493867 @default.
- W2014649079 hasConcept C86803240 @default.
- W2014649079 hasConcept C94412978 @default.
- W2014649079 hasConceptScore W2014649079C100544194 @default.
- W2014649079 hasConceptScore W2014649079C116174643 @default.
- W2014649079 hasConceptScore W2014649079C134643618 @default.
- W2014649079 hasConceptScore W2014649079C141603559 @default.
- W2014649079 hasConceptScore W2014649079C150903083 @default.
- W2014649079 hasConceptScore W2014649079C181199279 @default.
- W2014649079 hasConceptScore W2014649079C185592680 @default.
- W2014649079 hasConceptScore W2014649079C2775859485 @default.
- W2014649079 hasConceptScore W2014649079C2779920640 @default.
- W2014649079 hasConceptScore W2014649079C2780294150 @default.
- W2014649079 hasConceptScore W2014649079C27911776 @default.
- W2014649079 hasConceptScore W2014649079C31903555 @default.
- W2014649079 hasConceptScore W2014649079C55493867 @default.
- W2014649079 hasConceptScore W2014649079C86803240 @default.
- W2014649079 hasConceptScore W2014649079C94412978 @default.
- W2014649079 hasIssue "4" @default.
- W2014649079 hasLocation W20146490791 @default.
- W2014649079 hasLocation W20146490792 @default.
- W2014649079 hasOpenAccess W2014649079 @default.
- W2014649079 hasPrimaryLocation W20146490791 @default.
- W2014649079 hasRelatedWork W1965906019 @default.
- W2014649079 hasRelatedWork W2014649079 @default.
- W2014649079 hasRelatedWork W2089372297 @default.
- W2014649079 hasRelatedWork W2114464072 @default.
- W2014649079 hasRelatedWork W2144958090 @default.
- W2014649079 hasRelatedWork W2381615944 @default.
- W2014649079 hasRelatedWork W2393713774 @default.
- W2014649079 hasRelatedWork W2766313799 @default.
- W2014649079 hasRelatedWork W3161860268 @default.
- W2014649079 hasRelatedWork W3187000550 @default.
- W2014649079 hasVolume "24" @default.