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• W2014667686 abstract "Abstract The thiazol dye Thioflavin T (ThT), which is used to stain amyloid fibrils, was found to have strong inhibitory effects on both growth and conidiation of the deuteromycete Trichoderma viride at concentrations between 10–100 µg/ml (ca. 30–300 µmol/l). Thioflavin S (ThS), also known to stain amyloid fibrils, had no significant effect at these concentrations. Both stains yielded a fluorescence response, but their distributions were different. ThT was non-homogenously distributed throughout the cytoplasm, whereas ThS fluorescence was strongly bound to septal regions. The effect of ThT was studied on several model microorganisms. It exerted a strong inhibitory effect on Staphylococcus aureus (Gram-positive bacterium) (MIC=10 µmol/l), but the effect on Escherichia coli (Gram-negative bacterium) was one order of magnitude less pronounced. The effect on Candida albicans was also very strong (MIC=50 µmol/l). The dermatophytic fungus Microsporum gypseum and deuteromycete Alternaria alternata were less affected by ThT (MIC=250 µmol/l and >500 µmol/l, respectively). These results show that ThT could be a useful inhibitor of selected microorganisms, whereas ThS could be a useful agent for monitoring formation and maintenance of intrahyphal septa without inhibiting the growth of the microorganism." @default.
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• W2014667686 date "2010-03-03" @default.
• W2014667686 modified "2023-10-17" @default.
• W2014667686 title "New effects and applications of thioflavins" @default.
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• W2014667686 doi "https://doi.org/10.2478/s11535-010-0008-2" @default.
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