FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014690123> ?p ?o ?g. }

• W2014690123 endingPage "275" @default.
• W2014690123 startingPage "255" @default.
• W2014690123 abstract "Gene silencing in liver disease could be achieved by delivering siRNA with nonviral vectors. However, the transfection efficiency of plasmid siRNA ( psiRNA) applied through this approach in hepatocytes is generally low. Based on the fact that the asialoglycoprotein receptors present on hepatocytes can recognize galactose, we synthesized galactosylated poly(ethylene glycol)-graft-polyethylenimine (Gal-PEG-PEI) as a nonviral psiRNA carrier for hepatocyte targeting. Our results indicate that 0.2% (molar percentage) of amine groups of PEI was conjugated with PEG having galactose on its distal end. Increasing the molar ratios of Gal-PEG-PEI to psiRNA in complexation led to a decrease in particle size but an increase in zeta potential of complexes. The transfection efficiency of nanocomplexes, that is, Gal-PEG-PEI/psiRNA, in HepG2 cell line depends on the N/P value, which reflects the molar ratio of Gal-PEG-PEI to psiRNA in the complex. The highest transfection efficiency was 37.34%, which was obtained at N/P 8. At the same N/P value, the transfection efficiency with the nontargeting PEG-PEI/ psiRNA or Lipofectamine 2000/ psiRNA was much lower. The transfection efficiency of Gal-PEG-PEI/ psiRNA dropped to 3.60% from 37.34% after an excessive amount of free galactose was added into the medium for HepG2 cell incubation. By contrast, the similar phenomenon was observed neither when using PEG-PEI or Lipofectamine 2000 as a delivery vector nor in human embryonic kidney 293 cell line lacking ASGR. Real-time PCR analysis and western blot assay demonstrate that the knockdown of HLA-E gene expression by psiRNA/Gal-PEG-PEI (N/P 8) can reach about 60% in HepG2 cells after a 48-h transfection." @default.
• W2014690123 created "2016-06-24" @default.
• W2014690123 creator A5014998874 @default.
• W2014690123 creator A5016552002 @default.
• W2014690123 creator A5017521861 @default.
• W2014690123 creator A5019115888 @default.
• W2014690123 creator A5024686608 @default.
• W2014690123 creator A5041798817 @default.
• W2014690123 date "2010-05-28" @default.
• W2014690123 modified "2023-09-26" @default.
• W2014690123 title "Hepatocyte-targeted <i>psi</i>RNA Delivery Mediated by Galactosylated Poly(Ethylene Glycol)-Graft-Polyethylenimine <i>In Vitro</i>" @default.
• W2014690123 cites W1571612306 @default.
• W2014690123 cites W1605363717 @default.
• W2014690123 cites W1965658665 @default.
• W2014690123 cites W1969030848 @default.
• W2014690123 cites W1969532639 @default.
• W2014690123 cites W1970594627 @default.
• W2014690123 cites W1971162781 @default.
• W2014690123 cites W1979146520 @default.
• W2014690123 cites W1982968590 @default.
• W2014690123 cites W1992998779 @default.
• W2014690123 cites W1996643216 @default.
• W2014690123 cites W1999517163 @default.
• W2014690123 cites W2006604904 @default.
• W2014690123 cites W2009508594 @default.
• W2014690123 cites W2010655741 @default.
• W2014690123 cites W2016750917 @default.
• W2014690123 cites W2018879159 @default.
• W2014690123 cites W2041874034 @default.
• W2014690123 cites W2046663040 @default.
• W2014690123 cites W2049105846 @default.
• W2014690123 cites W2050085273 @default.
• W2014690123 cites W2061136666 @default.
• W2014690123 cites W2061195883 @default.
• W2014690123 cites W2061530113 @default.
• W2014690123 cites W2073725948 @default.
• W2014690123 cites W2081197509 @default.
• W2014690123 cites W2086580672 @default.
• W2014690123 cites W2087497808 @default.
• W2014690123 cites W2089006322 @default.
• W2014690123 cites W2089144046 @default.
• W2014690123 cites W2090114977 @default.
• W2014690123 cites W2093648430 @default.
• W2014690123 cites W2105878567 @default.
• W2014690123 cites W2107277218 @default.
• W2014690123 cites W2110622678 @default.
• W2014690123 cites W2128486910 @default.
• W2014690123 cites W2128712568 @default.
• W2014690123 cites W2135515894 @default.
• W2014690123 cites W2145430678 @default.
• W2014690123 cites W2161585699 @default.
• W2014690123 cites W2316709739 @default.
• W2014690123 doi "https://doi.org/10.1177/0885328210364678" @default.
• W2014690123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20511388" @default.
• W2014690123 hasPublicationYear "2010" @default.
• W2014690123 type Work @default.
• W2014690123 sameAs 2014690123 @default.
• W2014690123 citedByCount "17" @default.
• W2014690123 countsByYear W20146901232012 @default.
• W2014690123 countsByYear W20146901232013 @default.
• W2014690123 countsByYear W20146901232014 @default.
• W2014690123 countsByYear W20146901232015 @default.
• W2014690123 countsByYear W20146901232016 @default.
• W2014690123 countsByYear W20146901232017 @default.
• W2014690123 countsByYear W20146901232018 @default.
• W2014690123 countsByYear W20146901232020 @default.
• W2014690123 crossrefType "journal-article" @default.
• W2014690123 hasAuthorship W2014690123A5014998874 @default.
• W2014690123 hasAuthorship W2014690123A5016552002 @default.
• W2014690123 hasAuthorship W2014690123A5017521861 @default.
• W2014690123 hasAuthorship W2014690123A5019115888 @default.
• W2014690123 hasAuthorship W2014690123A5024686608 @default.
• W2014690123 hasAuthorship W2014690123A5041798817 @default.
• W2014690123 hasConcept C10138342 @default.
• W2014690123 hasConcept C104317684 @default.
• W2014690123 hasConcept C11569505 @default.
• W2014690123 hasConcept C135983454 @default.
• W2014690123 hasConcept C153911025 @default.
• W2014690123 hasConcept C162324750 @default.
• W2014690123 hasConcept C178790620 @default.
• W2014690123 hasConcept C185592680 @default.
• W2014690123 hasConcept C202751555 @default.
• W2014690123 hasConcept C2775925850 @default.
• W2014690123 hasConcept C2776200302 @default.
• W2014690123 hasConcept C2777516009 @default.
• W2014690123 hasConcept C40767141 @default.
• W2014690123 hasConcept C49923114 @default.
• W2014690123 hasConcept C54009773 @default.
• W2014690123 hasConcept C54400483 @default.
• W2014690123 hasConcept C55493867 @default.
• W2014690123 hasConcept C86803240 @default.
• W2014690123 hasConcept C92087593 @default.
• W2014690123 hasConceptScore W2014690123C10138342 @default.
• W2014690123 hasConceptScore W2014690123C104317684 @default.
• W2014690123 hasConceptScore W2014690123C11569505 @default.
• W2014690123 hasConceptScore W2014690123C135983454 @default.
• W2014690123 hasConceptScore W2014690123C153911025 @default.
• W2014690123 hasConceptScore W2014690123C162324750 @default.

• next