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- W2014711098 abstract "Abstract A simple and precise method for quantitatively estimating basophilia of tissue cultures, tissue sections or cells in suspension has been evolved. This consists of staining cells in a standard way with methylene blue, eluting the dye and estimating it colorimetrically. Experiments show that 90 per cent of the bound dye is associated with nucleic acids and that dye-binding parallels nucleic acid content. Nevertheless, the technique does not provide an accurate estimate of nucleic acid content. In growing cultures the dye bound does not necessarily correspond with cell number so that the technique has only limited application as an index of cell multiplication but may prove useful in screening tests." @default.
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- W2014711098 date "1960-06-01" @default.
- W2014711098 modified "2023-09-27" @default.
- W2014711098 title "Quantitative studies on the absorption and elution of methylene blue" @default.
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- W2014711098 doi "https://doi.org/10.1016/0014-4827(60)90227-5" @default.
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