FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014718653> ?p ?o ?g. }

• W2014718653 endingPage "1" @default.
• W2014718653 startingPage "1" @default.
• W2014718653 abstract "Background : Porcine endogenous retroviruses (PERVs) form part of the chromosomes of all pigs. Since they can be produced as infectious virion and infect human cells, safety issues on PERVs infection to human are still controversial and is one of main hurdles of xenotransplantation using pig cells or organs. It has been reported that the established porcine cell line, PK-15, produces PERVs and can infect the human cell lines. Therefore, clonal analysis on human cell line infected with PERV is a prerequisite to characterize the infectivity to human cells and to investigate the harmfulness of PERVs to human. Materials and Methods : For the characterization of PERV that originates from porcine cell line, PK-15, full length PERV cloning from genomic DNA of PK-15 was performed and partial sequences of both ends were achieved. Cell clones from human cell line, 293, persistently infected with PERVs from PK-15 were established by the method of limiting dilution. Nested PCR and direct sequencing of PCR products in each clone were carried out so as to confirm the PERV genomes in each clone. The growth rate of each clone was checked using cell counting and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, the infectivity by reverse transcriptase (RT) assay, and genetic analysis by karyotyping. Results :A total of 12 genomic PERV clones could be retrieved; 1 with full length, 4 with defective forms, and others with irrelevant sequences. Intact PERV was thought to be able to infect 293 and the PERV-infected cell clones were selected by limiting dilution. PCR results confirmed that nine cell clones were infected with PERV, and sequence alignment data on PCR products of pol region from PK-15 and human cell clones with PERV showed very similar results. Cell counting and MTT assay for growth kinetics of each clone indicated that two clones showed reduced growth rate. However, it was difficult to verify the effect of PERV infection on the cell growth because of the presence of many genetic alterations in 293 parental cells. No RT activities were detected in the culture supernatant from PERV-infected 293 cell clones. Conclusion : The sequences of PERVs were detected in human cell clones after PERV infection, but PERV virions could not be detected from the culture supernatant by RT assay." @default.
• W2014718653 created "2016-06-24" @default.
• W2014718653 creator A5014366855 @default.
• W2014718653 creator A5019954476 @default.
• W2014718653 creator A5028461178 @default.
• W2014718653 creator A5041546200 @default.
• W2014718653 creator A5045046458 @default.
• W2014718653 creator A5055753381 @default.
• W2014718653 creator A5073792718 @default.
• W2014718653 date "2009-01-01" @default.
• W2014718653 modified "2023-10-12" @default.
• W2014718653 title "Characterization of Clones of Human Cell Line Infected with Porcine Endogenous Retrovirus (PERV) from Porcine Cell Line, PK-15" @default.
• W2014718653 cites W1581414507 @default.
• W2014718653 cites W1967414895 @default.
• W2014718653 cites W2018180123 @default.
• W2014718653 cites W2020302113 @default.
• W2014718653 cites W2030869067 @default.
• W2014718653 cites W2039769323 @default.
• W2014718653 cites W2047235631 @default.
• W2014718653 cites W2056255385 @default.
• W2014718653 cites W2101868146 @default.
• W2014718653 cites W2109299179 @default.
• W2014718653 cites W2116120304 @default.
• W2014718653 cites W2130411738 @default.
• W2014718653 cites W2162217372 @default.
• W2014718653 cites W2168013364 @default.
• W2014718653 cites W3021782385 @default.
• W2014718653 doi "https://doi.org/10.3947/ic.2009.41.1.1" @default.
• W2014718653 hasPublicationYear "2009" @default.
• W2014718653 type Work @default.
• W2014718653 sameAs 2014718653 @default.
• W2014718653 citedByCount "8" @default.
• W2014718653 countsByYear W20147186532012 @default.
• W2014718653 countsByYear W20147186532014 @default.
• W2014718653 countsByYear W20147186532018 @default.
• W2014718653 crossrefType "journal-article" @default.
• W2014718653 hasAuthorship W2014718653A5014366855 @default.
• W2014718653 hasAuthorship W2014718653A5019954476 @default.
• W2014718653 hasAuthorship W2014718653A5028461178 @default.
• W2014718653 hasAuthorship W2014718653A5041546200 @default.
• W2014718653 hasAuthorship W2014718653A5045046458 @default.
• W2014718653 hasAuthorship W2014718653A5055753381 @default.
• W2014718653 hasAuthorship W2014718653A5073792718 @default.
• W2014718653 hasConcept C104317684 @default.
• W2014718653 hasConcept C121346293 @default.
• W2014718653 hasConcept C126322002 @default.
• W2014718653 hasConcept C141231307 @default.
• W2014718653 hasConcept C153911025 @default.
• W2014718653 hasConcept C159047783 @default.
• W2014718653 hasConcept C16613235 @default.
• W2014718653 hasConcept C2522874641 @default.
• W2014718653 hasConcept C2778431562 @default.
• W2014718653 hasConcept C54355233 @default.
• W2014718653 hasConcept C71924100 @default.
• W2014718653 hasConcept C81885089 @default.
• W2014718653 hasConcept C86803240 @default.
• W2014718653 hasConceptScore W2014718653C104317684 @default.
• W2014718653 hasConceptScore W2014718653C121346293 @default.
• W2014718653 hasConceptScore W2014718653C126322002 @default.
• W2014718653 hasConceptScore W2014718653C141231307 @default.
• W2014718653 hasConceptScore W2014718653C153911025 @default.
• W2014718653 hasConceptScore W2014718653C159047783 @default.
• W2014718653 hasConceptScore W2014718653C16613235 @default.
• W2014718653 hasConceptScore W2014718653C2522874641 @default.
• W2014718653 hasConceptScore W2014718653C2778431562 @default.
• W2014718653 hasConceptScore W2014718653C54355233 @default.
• W2014718653 hasConceptScore W2014718653C71924100 @default.
• W2014718653 hasConceptScore W2014718653C81885089 @default.
• W2014718653 hasConceptScore W2014718653C86803240 @default.
• W2014718653 hasIssue "1" @default.
• W2014718653 hasLocation W20147186531 @default.
• W2014718653 hasOpenAccess W2014718653 @default.
• W2014718653 hasPrimaryLocation W20147186531 @default.
• W2014718653 hasRelatedWork W1991126365 @default.
• W2014718653 hasRelatedWork W2034253571 @default.
• W2014718653 hasRelatedWork W2043335215 @default.
• W2014718653 hasRelatedWork W2061690707 @default.
• W2014718653 hasRelatedWork W2081421817 @default.
• W2014718653 hasRelatedWork W2088493091 @default.
• W2014718653 hasRelatedWork W2249470604 @default.
• W2014718653 hasRelatedWork W2314591304 @default.
• W2014718653 hasRelatedWork W2441200422 @default.
• W2014718653 hasRelatedWork W2464899582 @default.
• W2014718653 hasVolume "41" @default.
• W2014718653 isParatext "false" @default.
• W2014718653 isRetracted "false" @default.
• W2014718653 magId "2014718653" @default.
• W2014718653 workType "article" @default.