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- W2014718744 abstract "Incorporation of chemical probes into proteins is a powerful way to elucidate biological processes and to engineer novel function. Here we describe an approach that allows ligation of synthetic molecules to target proteins in an intracellular environment. A cellular protein is genetically tagged with one-half of a split intein. The complementary half is linked in vitro to the synthetic probe, and this fusion is delivered into cells using a transduction peptide. Association of the intein halves in the cytosol triggers protein trans-splicing, resulting in the ligation of the probe to the target protein through a peptide bond. This process is specific and applicable to cytosolic and integral membrane proteins. The technology should allow cellular proteins to be elaborated with a variety of abiotic probes." @default.
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- W2014718744 date "2003-05-22" @default.
- W2014718744 modified "2023-10-16" @default.
- W2014718744 title "Protein Semi-Synthesis in Living Cells" @default.
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- W2014718744 doi "https://doi.org/10.1021/ja034736i" @default.
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