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• W2014721743 abstract "Myocilin variants, localized to the olfactomedin (OLF) domain, are linked to early-onset, inherited forms of open-angle glaucoma. Disease-causing myocilin variants accumulate within trabecular meshwork cells instead of being secreted to the trabecular extracellular matrix of the eye. We hypothesize that, like in other diseases of protein misfolding, aggregation and downstream pathogenesis originate from the compromised thermal stability of mutant myocilins. In an expansion of our pilot study of four mutants, we compare 21 additional purified OLF variants by using a fluorescence stability assay and investigate the secondary structure of the most stable variants by circular dichroism. Variants with lower melting temperatures are correlated with earlier glaucoma diagnoses. The chemical chaperone trimethylamine N-oxide is capable of restoring the stability of most, but not all, variants to wild-type (WT) levels. Interestingly, three reported OLF disease variants, A427T, G246R, and A445V, exhibited properties indistinguishable from those of WT OLF, but an increased apparent aggregation propensity in vitro relative to that of WT OLF suggests that biophysical factors other than thermal stability, such as kinetics and unfolding pathways, may also be involved in myocilin glaucoma pathogenesis. Similarly, no changes from WT OLF stability and secondary structure were detected for three annotated single-nucleotide polymorphism variants. Our work provides the first quantitative demonstration of compromised stability among many identified OLF variants and places myocilin glaucoma in the context of other diseases of protein misfolding." @default.
• W2014721743 created "2016-06-24" @default.
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• W2014721743 creator A5036155438 @default.
• W2014721743 creator A5039211587 @default.
• W2014721743 creator A5075604712 @default.
• W2014721743 date "2011-06-09" @default.
• W2014721743 modified "2023-09-23" @default.
• W2014721743 title "The Stability of Myocilin Olfactomedin Domain Variants Provides New Insight into Glaucoma as a Protein Misfolding Disorder" @default.
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• W2014721743 doi "https://doi.org/10.1021/bi200231x" @default.
• W2014721743 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3128542" @default.
• W2014721743 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21612213" @default.
• W2014721743 hasPublicationYear "2011" @default.
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