Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014733004> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2014733004 endingPage "559" @default.
- W2014733004 startingPage "559" @default.
- W2014733004 abstract "Mineralisation of bone and cartilage is essential for skeletal development and function. We have previously reported a novel gene (PHOSPHO1); a member of the large haloacid dehalogenase superfamily of hydrolases which has an active site indicative of a phosphatase. Its high expression in skeletal tissues has led us to speculate that PHOSPHO1 may be involved in the mineralisation process. Therefore, in this study, we have determined that PHOSPHO1 is localized to sites of mineralisation in both cartilage and bone. Recombinant derived PHOSPHO1 protein was produced and affinity purified PHOSPHO1 antiserum was generated and used to immunostain a range of skeletal and soft avian tissues. In addition, PHOSPHO1 gene expression was determined in SaOS-2 and MG-63 osteoblast-like cells by RT-PCR. In diaphyseal cortical bone, immunohistochemistry localized PHOSPHO1 protein to the osteoid layer of the periosteum, forming surfaces of growing osteons, and newly formed osteocytes, whereas the endosteum and closed osteons were negative. In growth plate cartilage, immunoreactivity was limited to the early hypertrophic chondrocytes and the ossification groove of Ranvier. Cartilage remnants and trabecular bone within the primary spongiosa exhibited strong immunoreactivity on their mineralising surfaces. In 17-day-old embryonic calvaria, the osteoid present on the intramembranous and periosteal bone surfaces stained positively for PHOSPHO1. All soft tissues examined were negative. PHOSPHO1 gene expression was detected in mineralising SaOS-2 but not in the non-mineralising MG-63 osteoblast-like cells and gene expression levels were unchanged by dexamethasone, estradiol, 1,25-dihydroxyvitamin D3 or PTHrP treatment. Western analysis of chick growth plate cell lysate yielded bands (30.4 and 28.6 kD) corresponding to transcripts initiated at each of two possible initiation codons indicating the presence of alternative transcripts for PHOSPHO1 in growth cartilage. These results confirm that the PHOSPHO1 protein and gene expression profile is consistent with a role for PHOSPHO1 in bone and cartilage matrix mineralisation." @default.
- W2014733004 created "2016-06-24" @default.
- W2014733004 creator A5025181117 @default.
- W2014733004 creator A5035218865 @default.
- W2014733004 creator A5040463614 @default.
- W2014733004 creator A5055628541 @default.
- W2014733004 creator A5075980338 @default.
- W2014733004 creator A5080370677 @default.
- W2014733004 creator A5087895317 @default.
- W2014733004 date "1995-12-01" @default.
- W2014733004 modified "2023-09-25" @default.
- W2014733004 title "PTH and extracellular nucleotides synergistically induce c-fos expression in osteoblasts via distinct signal transduction pathways." @default.
- W2014733004 doi "https://doi.org/10.1016/8756-3282(96)87794-5" @default.
- W2014733004 hasPublicationYear "1995" @default.
- W2014733004 type Work @default.
- W2014733004 sameAs 2014733004 @default.
- W2014733004 citedByCount "1" @default.
- W2014733004 crossrefType "journal-article" @default.
- W2014733004 hasAuthorship W2014733004A5025181117 @default.
- W2014733004 hasAuthorship W2014733004A5035218865 @default.
- W2014733004 hasAuthorship W2014733004A5040463614 @default.
- W2014733004 hasAuthorship W2014733004A5055628541 @default.
- W2014733004 hasAuthorship W2014733004A5075980338 @default.
- W2014733004 hasAuthorship W2014733004A5080370677 @default.
- W2014733004 hasAuthorship W2014733004A5087895317 @default.
- W2014733004 hasConcept C105702510 @default.
- W2014733004 hasConcept C153911025 @default.
- W2014733004 hasConcept C160160445 @default.
- W2014733004 hasConcept C17938293 @default.
- W2014733004 hasConcept C181199279 @default.
- W2014733004 hasConcept C185592680 @default.
- W2014733004 hasConcept C188945261 @default.
- W2014733004 hasConcept C202751555 @default.
- W2014733004 hasConcept C203014093 @default.
- W2014733004 hasConcept C204232928 @default.
- W2014733004 hasConcept C2777674748 @default.
- W2014733004 hasConcept C2778260815 @default.
- W2014733004 hasConcept C2778842504 @default.
- W2014733004 hasConcept C2780550940 @default.
- W2014733004 hasConcept C2781403057 @default.
- W2014733004 hasConcept C4224716 @default.
- W2014733004 hasConcept C55493867 @default.
- W2014733004 hasConcept C81880429 @default.
- W2014733004 hasConcept C86803240 @default.
- W2014733004 hasConcept C95444343 @default.
- W2014733004 hasConceptScore W2014733004C105702510 @default.
- W2014733004 hasConceptScore W2014733004C153911025 @default.
- W2014733004 hasConceptScore W2014733004C160160445 @default.
- W2014733004 hasConceptScore W2014733004C17938293 @default.
- W2014733004 hasConceptScore W2014733004C181199279 @default.
- W2014733004 hasConceptScore W2014733004C185592680 @default.
- W2014733004 hasConceptScore W2014733004C188945261 @default.
- W2014733004 hasConceptScore W2014733004C202751555 @default.
- W2014733004 hasConceptScore W2014733004C203014093 @default.
- W2014733004 hasConceptScore W2014733004C204232928 @default.
- W2014733004 hasConceptScore W2014733004C2777674748 @default.
- W2014733004 hasConceptScore W2014733004C2778260815 @default.
- W2014733004 hasConceptScore W2014733004C2778842504 @default.
- W2014733004 hasConceptScore W2014733004C2780550940 @default.
- W2014733004 hasConceptScore W2014733004C2781403057 @default.
- W2014733004 hasConceptScore W2014733004C4224716 @default.
- W2014733004 hasConceptScore W2014733004C55493867 @default.
- W2014733004 hasConceptScore W2014733004C81880429 @default.
- W2014733004 hasConceptScore W2014733004C86803240 @default.
- W2014733004 hasConceptScore W2014733004C95444343 @default.
- W2014733004 hasIssue "6" @default.
- W2014733004 hasLocation W20147330041 @default.
- W2014733004 hasOpenAccess W2014733004 @default.
- W2014733004 hasPrimaryLocation W20147330041 @default.
- W2014733004 hasRelatedWork W1969805235 @default.
- W2014733004 hasRelatedWork W1987004605 @default.
- W2014733004 hasRelatedWork W2033017057 @default.
- W2014733004 hasRelatedWork W2046577115 @default.
- W2014733004 hasRelatedWork W2094969512 @default.
- W2014733004 hasRelatedWork W2098958335 @default.
- W2014733004 hasRelatedWork W2130607173 @default.
- W2014733004 hasRelatedWork W2151242261 @default.
- W2014733004 hasRelatedWork W2807924733 @default.
- W2014733004 hasRelatedWork W2953820071 @default.
- W2014733004 hasVolume "17" @default.
- W2014733004 isParatext "false" @default.
- W2014733004 isRetracted "false" @default.
- W2014733004 magId "2014733004" @default.
- W2014733004 workType "article" @default.