FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014736597> ?p ?o ?g. }

• W2014736597 endingPage "e106556" @default.
• W2014736597 startingPage "e106556" @default.
• W2014736597 abstract "Keratoconus (KC) is a complex thinning disease of the cornea that often requires transplantation. The underlying pathogenic molecular changes in this disease are poorly understood. Earlier studies reported oxidative stress, metabolic dysfunctions and accelerated death of stromal keratocytes in keratoconus (KC) patients. Utilizing mass spectrometry we found reduced stromal extracellular matrix (ECM) proteins in KC, suggesting ECM-regulatory changes that may be due to altered TGFβ signals. Here we investigated properties of stromal cells from donor (DN) and KC corneas grown as fibroblasts in serum containing DMEM: F12 or in serum-free medium containing insulin, transferrin, selenium (ITS). Phosphorylation of SMAD2/3 of the canonical TGFβ pathway, was high in serum-starved DN and KC fibroblast protein extracts, but pSMAD1/5/8 low at base line, was induced within 30 minutes of TGFβ1 stimulation, more so in KC than DN, suggesting a novel TGFβ1-SMAD1/5/8 axis in the cornea, that may be altered in KC. The serine/threonine kinases AKT, known to regulate proliferation, survival and biosynthetic activities of cells, were poorly activated in KC fibroblasts in high glucose media. Concordantly, alcohol dehydrogenase 1 (ADH1), an indicator of increased glucose uptake and metabolism, was reduced in KC compared to DN fibroblasts. By contrast, in low glucose (5.5 mM, normoglycemic) serum-free DMEM and ITS, cell survival and pAKT levels were comparable in KC and DN cells. Therefore, high glucose combined with serum-deprivation presents some cellular stress difficult to overcome by the KC stromal cells. Our study provides molecular insights into AKT and TGFβ signal changes in KC, and a mechanism for functional studies of stromal cells from KC corneas." @default.
• W2014736597 created "2016-06-24" @default.
• W2014736597 creator A5025812003 @default.
• W2014736597 creator A5034616780 @default.
• W2014736597 creator A5041652239 @default.
• W2014736597 creator A5048436248 @default.
• W2014736597 creator A5058974651 @default.
• W2014736597 creator A5059376643 @default.
• W2014736597 creator A5065678326 @default.
• W2014736597 creator A5067112714 @default.
• W2014736597 creator A5072038931 @default.
• W2014736597 date "2014-09-23" @default.
• W2014736597 modified "2023-10-06" @default.
• W2014736597 title "Transforming Growth Factor β and Insulin Signal Changes in Stromal Fibroblasts of Individual Keratoconus Patients" @default.
• W2014736597 cites W1571263028 @default.
• W2014736597 cites W1964923542 @default.
• W2014736597 cites W1968506526 @default.
• W2014736597 cites W1970098795 @default.
• W2014736597 cites W1970625951 @default.
• W2014736597 cites W1972061655 @default.
• W2014736597 cites W1972157351 @default.
• W2014736597 cites W1975681541 @default.
• W2014736597 cites W1978227510 @default.
• W2014736597 cites W1978708618 @default.
• W2014736597 cites W1979161589 @default.
• W2014736597 cites W1984621921 @default.
• W2014736597 cites W1992121419 @default.
• W2014736597 cites W1992910317 @default.
• W2014736597 cites W2004078164 @default.
• W2014736597 cites W2015112643 @default.
• W2014736597 cites W2016285900 @default.
• W2014736597 cites W2025073474 @default.
• W2014736597 cites W2028237347 @default.
• W2014736597 cites W2032350494 @default.
• W2014736597 cites W2041938961 @default.
• W2014736597 cites W2050682997 @default.
• W2014736597 cites W2052825248 @default.
• W2014736597 cites W2057645593 @default.
• W2014736597 cites W2064807768 @default.
• W2014736597 cites W2066938417 @default.
• W2014736597 cites W2068746373 @default.
• W2014736597 cites W2070033414 @default.
• W2014736597 cites W2070965714 @default.
• W2014736597 cites W2071341485 @default.
• W2014736597 cites W2071757683 @default.
• W2014736597 cites W2076188378 @default.
• W2014736597 cites W2077317914 @default.
• W2014736597 cites W2077575052 @default.
• W2014736597 cites W2078658792 @default.
• W2014736597 cites W2078667725 @default.
• W2014736597 cites W2086432076 @default.
• W2014736597 cites W2089148050 @default.
• W2014736597 cites W2097505959 @default.
• W2014736597 cites W2112980158 @default.
• W2014736597 cites W2113529261 @default.
• W2014736597 cites W2114826384 @default.
• W2014736597 cites W2115352199 @default.
• W2014736597 cites W2119016772 @default.
• W2014736597 cites W2121600519 @default.
• W2014736597 cites W2136374688 @default.
• W2014736597 cites W2141178367 @default.
• W2014736597 cites W2150548551 @default.
• W2014736597 cites W2159139120 @default.
• W2014736597 cites W2161153914 @default.
• W2014736597 cites W2164117279 @default.
• W2014736597 cites W2172043646 @default.
• W2014736597 cites W2225505855 @default.
• W2014736597 cites W4210991337 @default.
• W2014736597 cites W4295989308 @default.
• W2014736597 cites W48937670 @default.
• W2014736597 doi "https://doi.org/10.1371/journal.pone.0106556" @default.
• W2014736597 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4172437" @default.
• W2014736597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25247416" @default.
• W2014736597 hasPublicationYear "2014" @default.
• W2014736597 type Work @default.
• W2014736597 sameAs 2014736597 @default.
• W2014736597 citedByCount "21" @default.
• W2014736597 countsByYear W20147365972015 @default.
• W2014736597 countsByYear W20147365972016 @default.
• W2014736597 countsByYear W20147365972017 @default.
• W2014736597 countsByYear W20147365972018 @default.
• W2014736597 countsByYear W20147365972019 @default.
• W2014736597 countsByYear W20147365972020 @default.
• W2014736597 countsByYear W20147365972021 @default.
• W2014736597 countsByYear W20147365972022 @default.
• W2014736597 countsByYear W20147365972023 @default.
• W2014736597 crossrefType "journal-article" @default.
• W2014736597 hasAuthorship W2014736597A5025812003 @default.
• W2014736597 hasAuthorship W2014736597A5034616780 @default.
• W2014736597 hasAuthorship W2014736597A5041652239 @default.
• W2014736597 hasAuthorship W2014736597A5048436248 @default.
• W2014736597 hasAuthorship W2014736597A5058974651 @default.
• W2014736597 hasAuthorship W2014736597A5059376643 @default.
• W2014736597 hasAuthorship W2014736597A5065678326 @default.
• W2014736597 hasAuthorship W2014736597A5067112714 @default.
• W2014736597 hasAuthorship W2014736597A5072038931 @default.
• W2014736597 hasBestOaLocation W20147365971 @default.
• W2014736597 hasConcept C118131993 @default.

• next