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- W2014749500 abstract "Four-day primary coverglass cultures of rat heart muscle (M) and endothelioid (E) cells were treated for 30 min with stearic, oleic or linoleic acids in a FFA/albumin ratio of 6:1 at concentrations from 5 × 10−6 M to 5 × 10−4 M. Labilization of lysosomes and mitochondria was measured by acid phosphatase and succinic dehydrogenase staining activity respectively.Stearate or linoleate, but not oleate, labilized M cell lysosomes at 5 × 10−6 M. Lysosomes of E cells were not significantly affected by any of the FFA at 5 × 10−4 M. The order of activity of these FFA for M cell lysosomes was linoleate = stearate > oleate.Both E and M cell mitochondria were significantly labilized by oleate or linoleate, 5 × 10−6 M, and by stearate, 5 × 10−5 M. The order of activity was linoleate > oleate > stearate.Treatment of cultures for 24 hr with 50 μg/ml of hydrocortisone before the FFA at 5 × 10−5 M provided significant protection only against stearate-induced lysosomal labilization." @default.
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- W2014749500 date "1960-04-01" @default.
- W2014749500 modified "2023-10-11" @default.
- W2014749500 title "Permeability factors in the assay of mitochondrial dehydrogenases" @default.
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- W2014749500 doi "https://doi.org/10.1016/0006-291x(60)90183-2" @default.
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