FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014758210> ?p ?o ?g. }

• W2014758210 endingPage "753" @default.
• W2014758210 startingPage "731" @default.
• W2014758210 abstract "1. In darkness, a single rod outer segment isolated from the toad retina was sucked partially, tip first, into a tight-fitting, Ringer solution-filled glass pipette for recording membrane current. The basal end of the outer segment outside the pipette was sheared off with a probe to allow internal dialysis. The potential between the inside and the outside of the pipette was held at 0 mV. 2. With cyclic GMP and IBMX (isobutylmethylxanthine) in the dialysis solution, a large inward current appeared across the plasma membrane of the outer segment; this current saturated at around 1 mM-cyclic GMP. IBMX by itself was ineffective. 3. The saturated cyclic GMP-induced current recorded varied in size with the length of outer segment (L) within the suction pipette. For L less than 25 micron, the relation was linear, with a current density of 4-20 pA micron-1. 4. At short L (less than 25 micron), the dose-response relation between current magnitude and cyclic GMP concentration was sigmoidal, with a Hill coefficient (n) of 1.8-3.1 and a half-saturating cyclic GMP concentration (K1/2) of 30-85 microM. 5. In the presence of IBMX and the absence of GTP, the dose-response relation was the same in continuous bleaching light as in darkness. This indicates that both the characteristics of cyclic GMP binding and the intrinsic conduction properties of the open conductance are not affected by light. 6. Removing IBMX from the dialysing solution had little effect on the saturated current, but substantially reduced the current induced at low concentrations of cyclic GMP. When the analogue 8-bromo cyclic GMP was used instead, however, the presence of IBMX was relatively unimportant even at low agonist concentrations. These observations indicated that significant phosphodiesterase activity was present within the truncated outer segment. 7. In the absence of IBMX and the presence of GTP, the cyclic GMP-induced current could be suppressed by light. When ATP was also present in the dialysing solution, the effect of light was significantly reduced and the suppression also became more transient. 8. We conclude from the above results that the cyclic GMP-gated conductance is indeed present in the plasma membrane of the rod outer segment, and that this conductance and the light-sensitive conductance are one and the same entity. 9. From the results, we estimate that only about 1% of the conductance is normally open in darkness. This fraction of open conductance corresponds to a free cyclic GMP concentration of a few micromolar." @default.
• W2014758210 created "2016-06-24" @default.
• W2014758210 creator A5004855906 @default.
• W2014758210 creator A5082293289 @default.
• W2014758210 date "1988-01-01" @default.
• W2014758210 modified "2023-09-25" @default.
• W2014758210 title "Guanosine 3',5'-cyclic monophosphate-activated conductance studied in a truncated rod outer segment of the toad." @default.
• W2014758210 cites W1563310899 @default.
• W2014758210 cites W1590951841 @default.
• W2014758210 cites W1819679577 @default.
• W2014758210 cites W1969755572 @default.
• W2014758210 cites W1971663258 @default.
• W2014758210 cites W1974191268 @default.
• W2014758210 cites W1980862726 @default.
• W2014758210 cites W1997333309 @default.
• W2014758210 cites W1997771076 @default.
• W2014758210 cites W1998183048 @default.
• W2014758210 cites W2001072141 @default.
• W2014758210 cites W2008788879 @default.
• W2014758210 cites W2010415181 @default.
• W2014758210 cites W2019447882 @default.
• W2014758210 cites W2025364863 @default.
• W2014758210 cites W2029232890 @default.
• W2014758210 cites W2043633645 @default.
• W2014758210 cites W2045546014 @default.
• W2014758210 cites W2047057288 @default.
• W2014758210 cites W2059468094 @default.
• W2014758210 cites W2062384659 @default.
• W2014758210 cites W2077330062 @default.
• W2014758210 cites W2078987533 @default.
• W2014758210 cites W2079129494 @default.
• W2014758210 cites W2082873326 @default.
• W2014758210 cites W2083199107 @default.
• W2014758210 cites W2087056647 @default.
• W2014758210 cites W2087122651 @default.
• W2014758210 cites W2106781808 @default.
• W2014758210 cites W2113506485 @default.
• W2014758210 cites W2122577796 @default.
• W2014758210 cites W2123874680 @default.
• W2014758210 cites W2147005119 @default.
• W2014758210 cites W2147072462 @default.
• W2014758210 cites W2148070792 @default.
• W2014758210 cites W2162410350 @default.
• W2014758210 cites W2186882883 @default.
• W2014758210 cites W4237421066 @default.
• W2014758210 doi "https://doi.org/10.1113/jphysiol.1988.sp016943" @default.
• W2014758210 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1192018" @default.
• W2014758210 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2457686" @default.
• W2014758210 hasPublicationYear "1988" @default.
• W2014758210 type Work @default.
• W2014758210 sameAs 2014758210 @default.
• W2014758210 citedByCount "117" @default.
• W2014758210 countsByYear W20147582102012 @default.
• W2014758210 countsByYear W20147582102013 @default.
• W2014758210 countsByYear W20147582102018 @default.
• W2014758210 countsByYear W20147582102023 @default.
• W2014758210 crossrefType "journal-article" @default.
• W2014758210 hasAuthorship W2014758210A5004855906 @default.
• W2014758210 hasAuthorship W2014758210A5082293289 @default.
• W2014758210 hasBestOaLocation W20147582102 @default.
• W2014758210 hasConcept C105702510 @default.
• W2014758210 hasConcept C113196181 @default.
• W2014758210 hasConcept C114614502 @default.
• W2014758210 hasConcept C121932024 @default.
• W2014758210 hasConcept C12554922 @default.
• W2014758210 hasConcept C134018914 @default.
• W2014758210 hasConcept C147789679 @default.
• W2014758210 hasConcept C151552171 @default.
• W2014758210 hasConcept C170493617 @default.
• W2014758210 hasConcept C185592680 @default.
• W2014758210 hasConcept C192562407 @default.
• W2014758210 hasConcept C2778166480 @default.
• W2014758210 hasConcept C2779027399 @default.
• W2014758210 hasConcept C2779276759 @default.
• W2014758210 hasConcept C2781298581 @default.
• W2014758210 hasConcept C33923547 @default.
• W2014758210 hasConcept C43617362 @default.
• W2014758210 hasConcept C55493867 @default.
• W2014758210 hasConcept C59822182 @default.
• W2014758210 hasConcept C86803240 @default.
• W2014758210 hasConceptScore W2014758210C105702510 @default.
• W2014758210 hasConceptScore W2014758210C113196181 @default.
• W2014758210 hasConceptScore W2014758210C114614502 @default.
• W2014758210 hasConceptScore W2014758210C121932024 @default.
• W2014758210 hasConceptScore W2014758210C12554922 @default.
• W2014758210 hasConceptScore W2014758210C134018914 @default.
• W2014758210 hasConceptScore W2014758210C147789679 @default.
• W2014758210 hasConceptScore W2014758210C151552171 @default.
• W2014758210 hasConceptScore W2014758210C170493617 @default.
• W2014758210 hasConceptScore W2014758210C185592680 @default.
• W2014758210 hasConceptScore W2014758210C192562407 @default.
• W2014758210 hasConceptScore W2014758210C2778166480 @default.
• W2014758210 hasConceptScore W2014758210C2779027399 @default.
• W2014758210 hasConceptScore W2014758210C2779276759 @default.
• W2014758210 hasConceptScore W2014758210C2781298581 @default.
• W2014758210 hasConceptScore W2014758210C33923547 @default.
• W2014758210 hasConceptScore W2014758210C43617362 @default.
• W2014758210 hasConceptScore W2014758210C55493867 @default.

• next