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• W2014759273 abstract "The peptide ligase subtiligase, derived from subtilisin, has been employed in the identification of protein N-termini in complex mixtures. Here, the peptide ester substrates for the ligation reaction were optimized with respect to solubility, resulting in greater incorporation of the N-terminal tags. Additionally, the quantitation of the incorporated tags was explored, and a ‘click’ chemistry-based derivatization provided the ability to quantitate the tag to low nanomolar concentrations by sandwich ELISA. These new tags should expand the utility of subtiligase for the proteomic study of N-termini." @default.
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• W2014759273 date "2008-11-01" @default.
• W2014759273 modified "2023-09-26" @default.
• W2014759273 title "Tags for labeling protein N-termini with subtiligase for proteomics" @default.
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• W2014759273 doi "https://doi.org/10.1016/j.bmcl.2008.08.044" @default.
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