FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014765717> ?p ?o ?g. }

• W2014765717 endingPage "3376" @default.
• W2014765717 startingPage "3369" @default.
• W2014765717 abstract "Posttranslational glycosylation is critical for biological function of many proteins, but its structural characterization is complicated by natural heterogeneity, multiple glycosylation sites, and different forms. Here, a top-down mass spectrometry (MS) characterization is applied to three constructs of the Fc segment of IgE: Fcepsilon(3-4) (52 kDa) and Fcepsilon(2-3-4)(2) (76 kDa) disulfide-bonded homodimers. Fourier transform MS of a reduced sample of Fcepsilon(2-3-4) gave molecular masses of 37 527, 37 689, 37 851, and 38 014 Da, directly characterizing multiple glycoforms (hexose = 162 Da) without chromatographic separation. Limited proteolysis of the nonreduced Fcepsilon(2-3-4)(2) protein yielded a peptide mixture with molecular weight values that agreed with those expected from the DNA sequence. The single glycosylation site in these constructs was identified, and quantities were determined of five glycoforms that agreed within +/-2% of the molecular ion values. The 2-D mass spectrum of two glycosylated peptides showed these to have high-mannose structures, -GlcNAc-(hex)(n)(), demonstrating that Fcepsilon(2-3-4) has a single such structure of n = 5-9. For a mutated sample of Fcepsilon(3-4), in addition to five glycoforms, MS showed a molecular discrepancy that could be assigned with proteolysis and 2-D mass spectra to the oxidation of two methionines and an additional residue difference." @default.
• W2014765717 created "2016-06-24" @default.
• W2014765717 creator A5002939449 @default.
• W2014765717 creator A5011626912 @default.
• W2014765717 creator A5025344866 @default.
• W2014765717 creator A5028893802 @default.
• W2014765717 creator A5047693717 @default.
• W2014765717 creator A5048526342 @default.
• W2014765717 date "2000-02-26" @default.
• W2014765717 modified "2023-10-12" @default.
• W2014765717 title "Heterogeneous Glycosylation of Immunoglobulin E Constructs Characterized by Top-Down High-Resolution 2-D Mass Spectrometry" @default.
• W2014765717 cites W1268667419 @default.
• W2014765717 cites W1504726355 @default.
• W2014765717 cites W1966625877 @default.
• W2014765717 cites W1977291445 @default.
• W2014765717 cites W1991207270 @default.
• W2014765717 cites W1998922103 @default.
• W2014765717 cites W2026958168 @default.
• W2014765717 cites W2032388683 @default.
• W2014765717 cites W2034530880 @default.
• W2014765717 cites W2037549368 @default.
• W2014765717 cites W2069911595 @default.
• W2014765717 cites W2070070180 @default.
• W2014765717 cites W2073155155 @default.
• W2014765717 cites W2077241451 @default.
• W2014765717 cites W2079174216 @default.
• W2014765717 cites W2087399787 @default.
• W2014765717 cites W2091538590 @default.
• W2014765717 cites W2110812878 @default.
• W2014765717 cites W2121612064 @default.
• W2014765717 doi "https://doi.org/10.1021/bi9919091" @default.
• W2014765717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10727230" @default.
• W2014765717 hasPublicationYear "2000" @default.
• W2014765717 type Work @default.
• W2014765717 sameAs 2014765717 @default.
• W2014765717 citedByCount "60" @default.
• W2014765717 countsByYear W20147657172012 @default.
• W2014765717 countsByYear W20147657172013 @default.
• W2014765717 countsByYear W20147657172014 @default.
• W2014765717 countsByYear W20147657172015 @default.
• W2014765717 countsByYear W20147657172017 @default.
• W2014765717 countsByYear W20147657172018 @default.
• W2014765717 countsByYear W20147657172019 @default.
• W2014765717 countsByYear W20147657172023 @default.
• W2014765717 crossrefType "journal-article" @default.
• W2014765717 hasAuthorship W2014765717A5002939449 @default.
• W2014765717 hasAuthorship W2014765717A5011626912 @default.
• W2014765717 hasAuthorship W2014765717A5025344866 @default.
• W2014765717 hasAuthorship W2014765717A5028893802 @default.
• W2014765717 hasAuthorship W2014765717A5047693717 @default.
• W2014765717 hasAuthorship W2014765717A5048526342 @default.
• W2014765717 hasConcept C108625454 @default.
• W2014765717 hasConcept C162356407 @default.
• W2014765717 hasConcept C181199279 @default.
• W2014765717 hasConcept C185592680 @default.
• W2014765717 hasConcept C206212055 @default.
• W2014765717 hasConcept C2776393687 @default.
• W2014765717 hasConcept C2777313579 @default.
• W2014765717 hasConcept C2779281246 @default.
• W2014765717 hasConcept C2781307694 @default.
• W2014765717 hasConcept C2781338088 @default.
• W2014765717 hasConcept C30324644 @default.
• W2014765717 hasConcept C43617362 @default.
• W2014765717 hasConcept C55493867 @default.
• W2014765717 hasConcept C58121356 @default.
• W2014765717 hasConceptScore W2014765717C108625454 @default.
• W2014765717 hasConceptScore W2014765717C162356407 @default.
• W2014765717 hasConceptScore W2014765717C181199279 @default.
• W2014765717 hasConceptScore W2014765717C185592680 @default.
• W2014765717 hasConceptScore W2014765717C206212055 @default.
• W2014765717 hasConceptScore W2014765717C2776393687 @default.
• W2014765717 hasConceptScore W2014765717C2777313579 @default.
• W2014765717 hasConceptScore W2014765717C2779281246 @default.
• W2014765717 hasConceptScore W2014765717C2781307694 @default.
• W2014765717 hasConceptScore W2014765717C2781338088 @default.
• W2014765717 hasConceptScore W2014765717C30324644 @default.
• W2014765717 hasConceptScore W2014765717C43617362 @default.
• W2014765717 hasConceptScore W2014765717C55493867 @default.
• W2014765717 hasConceptScore W2014765717C58121356 @default.
• W2014765717 hasIssue "12" @default.
• W2014765717 hasLocation W20147657171 @default.
• W2014765717 hasLocation W20147657172 @default.
• W2014765717 hasOpenAccess W2014765717 @default.
• W2014765717 hasPrimaryLocation W20147657171 @default.
• W2014765717 hasRelatedWork W1712481278 @default.
• W2014765717 hasRelatedWork W1977314232 @default.
• W2014765717 hasRelatedWork W1990560603 @default.
• W2014765717 hasRelatedWork W2017264771 @default.
• W2014765717 hasRelatedWork W2092588194 @default.
• W2014765717 hasRelatedWork W2102332686 @default.
• W2014765717 hasRelatedWork W2139866700 @default.
• W2014765717 hasRelatedWork W2258351620 @default.
• W2014765717 hasRelatedWork W2974383127 @default.
• W2014765717 hasRelatedWork W4362636723 @default.
• W2014765717 hasVolume "39" @default.
• W2014765717 isParatext "false" @default.
• W2014765717 isRetracted "false" @default.
• W2014765717 magId "2014765717" @default.

• next