Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014769520> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2014769520 abstract "Skeletal muscles produce low levels of reactive oxygen species (ROS) under resting conditions while during contractile activity the rate of ROS production increases. Low levels of ROS production may act to stimulate adaptive responses in skeletal muscle, while increased ROS dependent oxidation at sarcoplasmic reticulum, myofilaments and other EC coupling components likely lead to decrements in contractile function. Multiple sites exist for ROS production, including mitochondria and NADPH oxidase; however, the contribution of each of these and the factors that regulate the increased production of ROS during contractile activity remains to be determined. Here we use repetitive field stimulation of single FDB myofibers as a model of ROS secondary to repetitive activity. In FDB's loaded with the cytosolic, non-specific ROS probe DCFH, we have imposed intermittent (0.5 Hz) trains (150msec, 0.5msec sq. pulse @, 50Hz) of tetanic stimulation to establish a reliable in vitro model for activity dependent ROS production. In recent studies with this model, we have begun to explore site dependent generation of ROS with a redox sensitive variant of green fluorescent protein (roGFP) that is targeted to the mitochondria (mito-roGFP). Following cDNA electroporation in vivo, expression of mito-roGFP, and FDB isolation, we report evidence of the fidelity and specificity of this probe in mitochondria and the activity dependent mitochondrial redox status during our stimulation paradigm." @default.
- W2014769520 created "2016-06-24" @default.
- W2014769520 creator A5010147367 @default.
- W2014769520 creator A5015992429 @default.
- W2014769520 creator A5041736581 @default.
- W2014769520 date "2009-02-01" @default.
- W2014769520 modified "2023-09-30" @default.
- W2014769520 title "Local Detection Of Intracellular Reactive Oxygen Species In Single Intact Contracting Skeletal Muscle Fibers" @default.
- W2014769520 doi "https://doi.org/10.1016/j.bpj.2008.12.1121" @default.
- W2014769520 hasPublicationYear "2009" @default.
- W2014769520 type Work @default.
- W2014769520 sameAs 2014769520 @default.
- W2014769520 citedByCount "0" @default.
- W2014769520 crossrefType "journal-article" @default.
- W2014769520 hasAuthorship W2014769520A5010147367 @default.
- W2014769520 hasAuthorship W2014769520A5015992429 @default.
- W2014769520 hasAuthorship W2014769520A5041736581 @default.
- W2014769520 hasBestOaLocation W20147695201 @default.
- W2014769520 hasConcept C105702510 @default.
- W2014769520 hasConcept C12554922 @default.
- W2014769520 hasConcept C134018914 @default.
- W2014769520 hasConcept C158617107 @default.
- W2014769520 hasConcept C181199279 @default.
- W2014769520 hasConcept C185592680 @default.
- W2014769520 hasConcept C24998067 @default.
- W2014769520 hasConcept C2778175917 @default.
- W2014769520 hasConcept C2779719074 @default.
- W2014769520 hasConcept C2779959927 @default.
- W2014769520 hasConcept C2780795997 @default.
- W2014769520 hasConcept C28859421 @default.
- W2014769520 hasConcept C48349386 @default.
- W2014769520 hasConcept C55493867 @default.
- W2014769520 hasConcept C79879829 @default.
- W2014769520 hasConcept C86803240 @default.
- W2014769520 hasConcept C95444343 @default.
- W2014769520 hasConcept C98539663 @default.
- W2014769520 hasConceptScore W2014769520C105702510 @default.
- W2014769520 hasConceptScore W2014769520C12554922 @default.
- W2014769520 hasConceptScore W2014769520C134018914 @default.
- W2014769520 hasConceptScore W2014769520C158617107 @default.
- W2014769520 hasConceptScore W2014769520C181199279 @default.
- W2014769520 hasConceptScore W2014769520C185592680 @default.
- W2014769520 hasConceptScore W2014769520C24998067 @default.
- W2014769520 hasConceptScore W2014769520C2778175917 @default.
- W2014769520 hasConceptScore W2014769520C2779719074 @default.
- W2014769520 hasConceptScore W2014769520C2779959927 @default.
- W2014769520 hasConceptScore W2014769520C2780795997 @default.
- W2014769520 hasConceptScore W2014769520C28859421 @default.
- W2014769520 hasConceptScore W2014769520C48349386 @default.
- W2014769520 hasConceptScore W2014769520C55493867 @default.
- W2014769520 hasConceptScore W2014769520C79879829 @default.
- W2014769520 hasConceptScore W2014769520C86803240 @default.
- W2014769520 hasConceptScore W2014769520C95444343 @default.
- W2014769520 hasConceptScore W2014769520C98539663 @default.
- W2014769520 hasLocation W20147695201 @default.
- W2014769520 hasOpenAccess W2014769520 @default.
- W2014769520 hasPrimaryLocation W20147695201 @default.
- W2014769520 hasRelatedWork W1583752057 @default.
- W2014769520 hasRelatedWork W1970576380 @default.
- W2014769520 hasRelatedWork W1980062647 @default.
- W2014769520 hasRelatedWork W2026660649 @default.
- W2014769520 hasRelatedWork W2035019480 @default.
- W2014769520 hasRelatedWork W2035431917 @default.
- W2014769520 hasRelatedWork W2040327999 @default.
- W2014769520 hasRelatedWork W2046490162 @default.
- W2014769520 hasRelatedWork W2050606684 @default.
- W2014769520 hasRelatedWork W2063704783 @default.
- W2014769520 hasRelatedWork W2076816233 @default.
- W2014769520 hasRelatedWork W2119213762 @default.
- W2014769520 hasRelatedWork W2317004990 @default.
- W2014769520 hasRelatedWork W2752033242 @default.
- W2014769520 hasRelatedWork W2767543684 @default.
- W2014769520 hasRelatedWork W2767862668 @default.
- W2014769520 hasRelatedWork W2802543878 @default.
- W2014769520 hasRelatedWork W2950574222 @default.
- W2014769520 hasRelatedWork W2953768338 @default.
- W2014769520 hasRelatedWork W2972080217 @default.
- W2014769520 isParatext "false" @default.
- W2014769520 isRetracted "false" @default.
- W2014769520 magId "2014769520" @default.
- W2014769520 workType "article" @default.