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- W2014774218 abstract "Field emission scanning electron microscopy (FESEM) preparation techniques have been successfully adapted for visualization of the internal and external ultrastructure of Mougeotia filaments and protoplasts. FESEM of the innermost layer of cell wall in Mougeotia filaments revealed that microfibrils are deposited parallel to each other in an interconnected mesh and are oriented perpendicular to the direction of elongation. For the first time, the surface of protoplasts at different stages of regeneration has been observed using FESEM. Nascent microfibril deposition occurs between 1 and 2 h after isolation and arrangement of these microfibrils is random for at least 8 h. Observation of the inner surface of the plasma membrane in burst protoplasts showed that microtubules are not strongly attached for at least 3 h after protoplast isolation." @default.
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- W2014774218 date "2002-09-09" @default.
- W2014774218 modified "2023-09-24" @default.
- W2014774218 title "High-resolution microscopy of cell wall formation in regeneratingMougeotia(Chlorophyceae) protoplasts" @default.
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- W2014774218 doi "https://doi.org/10.1017/s096702620200375x" @default.
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