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• W2014775978 abstract "The introduction of exogenous DNA into Escherichia coli is a cornerstone of molecular biology. Herein, we investigate two new mechanisms for bacterial transformation involving either the use of microwave irradiation or a freeze–thaw protocol in liquid nitrogen. Ultimately, both methods afforded successful transfer of plasmid DNA into bacterial cells, with the freeze–thaw technique yielding efficiencies of ~105. More importantly, both techniques effectively eliminated the need for the preparation of competent cells." @default.
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• W2014775978 date "2013-05-21" @default.
• W2014775978 modified "2023-09-26" @default.
• W2014775978 title "Development of rapid microwave-mediated and low-temperature bacterial transformations" @default.
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• W2014775978 doi "https://doi.org/10.1007/s12154-013-0095-4" @default.
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