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- W2014802721 abstract "Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein." @default.
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- W2014802721 date "1982-02-01" @default.
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- W2014802721 title "In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides." @default.
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- W2014802721 doi "https://doi.org/10.1073/pnas.79.3.754" @default.
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