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- W2014810050 abstract "Abstract The use of lipids in the construction of membranes for biosensors has been well documented. They have been used on electrode surfaces to reduce interferences from electroactive species such as ascorbate, while lipid bilayers have been used to immobilize enzymes. Recently, liposomes have been used to encapsulate glucose oxidase and these liposomes have been immobilized on a Rank electrode polarized to measure the production of hydrogen peroxide. In this case liposomes were used as a diffusion limiting membrane to reduce the exposure of the oxidase to glucose and therefore increase the linear range of the enzyme to a level of clinical use (0–40 mM). The original method of liposime production (reverse phase evaporation) was altered to a method of vesible extrusion to provide more reproducible results. Liposomal electrodes from this system showed an extended K m of over 100 mM and a slow response time of 5 min. This paper describes research undertaken on the manipulation of the lipid bilayer of the liposome. By varying the composition of the lipids in the bilayer the response size, time and apparent K m can all be altered." @default.
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- W2014810050 date "1997-01-01" @default.
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- W2014810050 title "The effect of lipid bilayer manipulation on the response of the glucose oxidase-liposome electrode" @default.
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- W2014810050 doi "https://doi.org/10.1016/s0956-5663(97)00001-8" @default.
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