FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014829450> ?p ?o ?g. }

• W2014829450 endingPage "583" @default.
• W2014829450 startingPage "572" @default.
• W2014829450 abstract "Suppression of macroautophagy, due to mutations or through processes linked to aging, results in the accumulation of cytoplasmic substrates that are normally eliminated by the pathway. This is a significant problem in long-lived cells like neurons, where pathway defects can result in the accumulation of aggregates containing ubiquitinated proteins. The p62/Ref(2)P family of proteins is involved in the autophagic clearance of cytoplasmic protein bodies or sequestosomes. These unique structures are closely associated with protein inclusions containing ubiquitin as well as key components of the autophagy pathway. In this study we show that detergent fractionation followed by western blot analysis of insoluble ubiquitinated proteins (IUP), mammalian p62 and its Drosophila homologue, Ref(2)P can be used to quantitatively assess the activity level of aggregate clearance (aggrephagy) in complex tissues. Using this technique we show that genetic or age-dependent changes that modify the long-term enhancement or suppression of aggrephagy can be identified. Moreover, using the Drosophila model system this method can be used to establish autophagy-dependent protein clearance profiles that are occurring under a wide range of physiological conditions including developmental, fasting and altered metabolic pathways. This technique can also be used to examine proteopathies that are associated with human disorders such as frontotemporal dementia, Huntington and Alzheimer disease. Our findings indicate that measuring IUP profiles together with an assessment of p62/Ref(2)P proteins can be used as a screening or diagnostic tool to characterize genetic and age-dependent factors that alter the long-term function of autophagy and the clearance of protein aggregates occurring within complex tissues and cells." @default.
• W2014829450 created "2016-06-24" @default.
• W2014829450 creator A5003461044 @default.
• W2014829450 creator A5009352340 @default.
• W2014829450 creator A5017842202 @default.
• W2014829450 creator A5018385928 @default.
• W2014829450 creator A5020024991 @default.
• W2014829450 creator A5028968650 @default.
• W2014829450 creator A5032472041 @default.
• W2014829450 creator A5058804009 @default.
• W2014829450 creator A5071774128 @default.
• W2014829450 creator A5072742742 @default.
• W2014829450 creator A5073036493 @default.
• W2014829450 date "2011-06-01" @default.
• W2014829450 modified "2023-10-01" @default.
• W2014829450 title "p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects" @default.
• W2014829450 cites W1508661542 @default.
• W2014829450 cites W1543411779 @default.
• W2014829450 cites W1571208010 @default.
• W2014829450 cites W1919353 @default.
• W2014829450 cites W1967809393 @default.
• W2014829450 cites W1975326119 @default.
• W2014829450 cites W1977311503 @default.
• W2014829450 cites W1988146888 @default.
• W2014829450 cites W1989498124 @default.
• W2014829450 cites W1993996853 @default.
• W2014829450 cites W1994305081 @default.
• W2014829450 cites W1995042437 @default.
• W2014829450 cites W1997112179 @default.
• W2014829450 cites W2005503622 @default.
• W2014829450 cites W2008735323 @default.
• W2014829450 cites W2009125409 @default.
• W2014829450 cites W2010247270 @default.
• W2014829450 cites W2013215890 @default.
• W2014829450 cites W2014918593 @default.
• W2014829450 cites W2014957683 @default.
• W2014829450 cites W2022812113 @default.
• W2014829450 cites W2023994136 @default.
• W2014829450 cites W2026743296 @default.
• W2014829450 cites W2032377673 @default.
• W2014829450 cites W2034561234 @default.
• W2014829450 cites W2038736919 @default.
• W2014829450 cites W2047144539 @default.
• W2014829450 cites W2047683380 @default.
• W2014829450 cites W2048267629 @default.
• W2014829450 cites W2051344987 @default.
• W2014829450 cites W2052093947 @default.
• W2014829450 cites W2052578642 @default.
• W2014829450 cites W2053168923 @default.
• W2014829450 cites W2056388945 @default.
• W2014829450 cites W2057518766 @default.
• W2014829450 cites W2060579362 @default.
• W2014829450 cites W2068159429 @default.
• W2014829450 cites W2073318890 @default.
• W2014829450 cites W2073349608 @default.
• W2014829450 cites W2075570226 @default.
• W2014829450 cites W2081106067 @default.
• W2014829450 cites W2083919459 @default.
• W2014829450 cites W2091068969 @default.
• W2014829450 cites W2092859042 @default.
• W2014829450 cites W2097631517 @default.
• W2014829450 cites W2101596944 @default.
• W2014829450 cites W2102354517 @default.
• W2014829450 cites W2103275390 @default.
• W2014829450 cites W2106195012 @default.
• W2014829450 cites W2111567059 @default.
• W2014829450 cites W2113892331 @default.
• W2014829450 cites W2115742671 @default.
• W2014829450 cites W2119399459 @default.
• W2014829450 cites W2126118460 @default.
• W2014829450 cites W2128101513 @default.
• W2014829450 cites W2128537698 @default.
• W2014829450 cites W2135686999 @default.
• W2014829450 cites W2138327656 @default.
• W2014829450 cites W2139425230 @default.
• W2014829450 cites W2143122495 @default.
• W2014829450 cites W2144103349 @default.
• W2014829450 cites W2145149844 @default.
• W2014829450 cites W2148013240 @default.
• W2014829450 cites W2154404753 @default.
• W2014829450 cites W2156228892 @default.
• W2014829450 cites W2165666114 @default.
• W2014829450 cites W2166124333 @default.
• W2014829450 cites W2167500696 @default.
• W2014829450 cites W2171771398 @default.
• W2014829450 cites W47168186 @default.
• W2014829450 doi "https://doi.org/10.4161/auto.7.6.14943" @default.
• W2014829450 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3127048" @default.
• W2014829450 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21325881" @default.
• W2014829450 hasPublicationYear "2011" @default.
• W2014829450 type Work @default.
• W2014829450 sameAs 2014829450 @default.
• W2014829450 citedByCount "167" @default.
• W2014829450 countsByYear W20148294502012 @default.
• W2014829450 countsByYear W20148294502013 @default.
• W2014829450 countsByYear W20148294502014 @default.
• W2014829450 countsByYear W20148294502015 @default.
• W2014829450 countsByYear W20148294502016 @default.

• next