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- W2014832613 abstract "Enzyme-linked immunosorbent assays were developed for two proteins found in mammary secretions during the non-lactating period: lactoferrin and a 39-kdal protein. Minimum detectable concentrations were .12 ng/ml for lactoferrin and .0037 ng/ml for the 39-kdal protein. Standard curves were linear in the ranges of .4 to 100 ng/ml for lactoferrin and 2.4 to 30 ng/ml for the 39-kdal protein. The immunoassays were used to measure concentrations of the proteins in mammary secretions during involution. From the last day of milking to d 30 of involution, mean concentrations of lactoferrin and the 39-kdal protein increased from .82 +/- .35 to 78.5 +/- 12.43 mg/ml and from 11.8 +/- 3.8 to 84.4 +/- 21.1 micrograms/ml, respectively. The immunoassays described will be valuable for further studies on the synthesis and secretion of these proteins during mammary involution." @default.
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- W2014832613 date "1989-02-01" @default.
- W2014832613 modified "2023-09-27" @default.
- W2014832613 title "Enzyme-Linked Immunosorbent Assays of Bovine Lactoferrin and a 39-Kilodalton Protein Found in Mammary Secretions During Involution" @default.
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- W2014832613 doi "https://doi.org/10.3168/jds.s0022-0302(89)79141-4" @default.
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