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- W2014863596 abstract "It has been assumed from the early work of Askew et al<sup>1</sup>and Windaus and co-workers<sup>2,3</sup>that vitamins D<sub>2</sub>and D<sub>3</sub>act directly in the organism without molecular change. Experiments by Kodicek<sup>4,5</sup>first demonstrated that vitamin D is in fact metabolized to biologically inactive break down products and lent further support to this assumption. In their hands, no evidence could be obtained for a biologically active metabolite of the vitamin. Clearly, their experiments were hindered by the low specific activity of vitamin D<sub>2</sub>labeled with radioactive carbon (<sup>14</sup>C) and by inadequate chromatographic methods so as to preclude the detection of a major biologically active metabolite. With the development of new and powerful chromatographic techniques<sup>6,7</sup>and the synthesis of a radioactive vitamin D preparation of high enough specific activity<sup>8-10</sup>to allow experiments with physiologic doses of the vitamin, the question of vitamin" @default.
- W2014863596 created "2016-06-24" @default.
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- W2014863596 date "1969-10-01" @default.
- W2014863596 modified "2023-10-04" @default.
- W2014863596 title "25-Hydroxycholecalciferol" @default.
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- W2014863596 doi "https://doi.org/10.1001/archinte.1969.00300200054009" @default.
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