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- W2014871318 abstract "The recombinant human tumour necrosis factor α from an extract of Escherichia coli was enriched to homogeneity according to specific activity and sodium dodecyl sulphate-polyacrylamide gel electrophoresis by purification using anion-exchange HPLC and hydrophobic interaction HPLC. Parallel experiments with the same separation methods, but carried out with membrane chromatography on compact discs, gave similar results in terms of yield and purity of the product. The active form of the protein is a trimer. The second isolation step, hydrophobic interaction chromatography, causes dissociation of the trimer into monomers and a partial loss of the biological activity of the protein. The phenomenon occurs on both the column and the disc. This in turn indicates strongly that the dissociation of the protein is a consequence of interaction between the sample and the hydrophobic ligand, and is not caused by non-specific interaction with the matrix." @default.
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- W2014871318 date "1994-02-01" @default.
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- W2014871318 title "Purification of human tumour necrosis factor by membrane chromatography" @default.
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- W2014871318 doi "https://doi.org/10.1016/0021-9673(94)85186-7" @default.
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