Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014896048> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2014896048 endingPage "12" @default.
- W2014896048 startingPage "1" @default.
- W2014896048 abstract "A reactive Glc analog, N-(bromoacetyl)-D-glucosamine (GlcNBrAc), has recently been used (D. M. Schirch and J. E. Wilson (1987) Arch. Biochem. Biophys. 254, 385-396) to label the Glc binding site of rat brain Type I hexokinase. This site has been located in a 40-kDa domain at the C-terminus of the enzyme previously shown to be the location of the substrate ATP binding site (M. Nemat-Gorgani and J. E. Wilson (1986) Arch. Biochem. Biophys. 251, 97-103). In the present study, peptide mapping of hexokinase modified by radiolabeled GlcNBrAc yields three labeled peptides (Peptides I-III). Peptides I and III, as well as catalytic activity, are protected by inclusion of Glc or GlcNAc during reaction with GlcNBrAc. These two peptides show considerable homology to contiguous regions in the sequences of yeast hexokinase isozymes A and B. Peptide III is homologous to a sequence which, based on the X-ray crystallographic work by Steitz and co-workers, is located near the Glc binding site of yeast hexokinase; Peptide I is homologous to an immediately adjacent (toward the C-terminus) region of yeast hexokinase. An essential serine residue implicated in the binding of Glc to the yeast enzyme is also conserved in Peptide III from rat brain hexokinase. These results provide strong support for the view that the catalytic domain at the C-terminus of the mammalian Type I hexokinase shares a common ancestry with yeast hexokinase. Peptide II appears to be nonspecifically labeled by GlcNBrAc since labeling is insensitive to the presence of protective ligands such as Glc or GlcNAc; the sequence of Peptide II shows no detectable homology with the yeast isozymes." @default.
- W2014896048 created "2016-06-24" @default.
- W2014896048 creator A5053877026 @default.
- W2014896048 creator A5086479392 @default.
- W2014896048 date "1987-08-01" @default.
- W2014896048 modified "2023-10-16" @default.
- W2014896048 title "Rat brain hexokinase: Amino acid sequence at the substrate hexose binding site is homologous to that of yeast hexokinase" @default.
- W2014896048 cites W1488554688 @default.
- W2014896048 cites W1498707166 @default.
- W2014896048 cites W1556781583 @default.
- W2014896048 cites W1565258648 @default.
- W2014896048 cites W1571793851 @default.
- W2014896048 cites W1576797769 @default.
- W2014896048 cites W1958797264 @default.
- W2014896048 cites W1966921308 @default.
- W2014896048 cites W1970881828 @default.
- W2014896048 cites W1973656618 @default.
- W2014896048 cites W1975944566 @default.
- W2014896048 cites W1978330903 @default.
- W2014896048 cites W1978902109 @default.
- W2014896048 cites W1984928080 @default.
- W2014896048 cites W1996209215 @default.
- W2014896048 cites W1996537553 @default.
- W2014896048 cites W2005558166 @default.
- W2014896048 cites W2015813546 @default.
- W2014896048 cites W2023881865 @default.
- W2014896048 cites W2030975188 @default.
- W2014896048 cites W2033662557 @default.
- W2014896048 cites W2044466662 @default.
- W2014896048 cites W2070506889 @default.
- W2014896048 cites W2084950970 @default.
- W2014896048 cites W2094219430 @default.
- W2014896048 cites W2120593135 @default.
- W2014896048 cites W2146646096 @default.
- W2014896048 cites W2150304477 @default.
- W2014896048 cites W2461190602 @default.
- W2014896048 cites W4238432345 @default.
- W2014896048 doi "https://doi.org/10.1016/0003-9861(87)90536-4" @default.
- W2014896048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3631958" @default.
- W2014896048 hasPublicationYear "1987" @default.
- W2014896048 type Work @default.
- W2014896048 sameAs 2014896048 @default.
- W2014896048 citedByCount "25" @default.
- W2014896048 crossrefType "journal-article" @default.
- W2014896048 hasAuthorship W2014896048A5053877026 @default.
- W2014896048 hasAuthorship W2014896048A5086479392 @default.
- W2014896048 hasConcept C104317684 @default.
- W2014896048 hasConcept C107824862 @default.
- W2014896048 hasConcept C119795356 @default.
- W2014896048 hasConcept C167625842 @default.
- W2014896048 hasConcept C181199279 @default.
- W2014896048 hasConcept C185592680 @default.
- W2014896048 hasConcept C20251656 @default.
- W2014896048 hasConcept C2776414213 @default.
- W2014896048 hasConcept C2777576037 @default.
- W2014896048 hasConcept C2779222958 @default.
- W2014896048 hasConcept C2779281246 @default.
- W2014896048 hasConcept C2779826832 @default.
- W2014896048 hasConcept C41183919 @default.
- W2014896048 hasConcept C55493867 @default.
- W2014896048 hasConcept C86803240 @default.
- W2014896048 hasConceptScore W2014896048C104317684 @default.
- W2014896048 hasConceptScore W2014896048C107824862 @default.
- W2014896048 hasConceptScore W2014896048C119795356 @default.
- W2014896048 hasConceptScore W2014896048C167625842 @default.
- W2014896048 hasConceptScore W2014896048C181199279 @default.
- W2014896048 hasConceptScore W2014896048C185592680 @default.
- W2014896048 hasConceptScore W2014896048C20251656 @default.
- W2014896048 hasConceptScore W2014896048C2776414213 @default.
- W2014896048 hasConceptScore W2014896048C2777576037 @default.
- W2014896048 hasConceptScore W2014896048C2779222958 @default.
- W2014896048 hasConceptScore W2014896048C2779281246 @default.
- W2014896048 hasConceptScore W2014896048C2779826832 @default.
- W2014896048 hasConceptScore W2014896048C41183919 @default.
- W2014896048 hasConceptScore W2014896048C55493867 @default.
- W2014896048 hasConceptScore W2014896048C86803240 @default.
- W2014896048 hasIssue "1" @default.
- W2014896048 hasLocation W20148960481 @default.
- W2014896048 hasLocation W20148960482 @default.
- W2014896048 hasOpenAccess W2014896048 @default.
- W2014896048 hasPrimaryLocation W20148960481 @default.
- W2014896048 hasRelatedWork W2018404331 @default.
- W2014896048 hasRelatedWork W2050182051 @default.
- W2014896048 hasRelatedWork W2062385823 @default.
- W2014896048 hasRelatedWork W2087346710 @default.
- W2014896048 hasRelatedWork W2146885685 @default.
- W2014896048 hasRelatedWork W2417920079 @default.
- W2014896048 hasRelatedWork W2467980817 @default.
- W2014896048 hasRelatedWork W4200459043 @default.
- W2014896048 hasRelatedWork W4283162294 @default.
- W2014896048 hasRelatedWork W903917906 @default.
- W2014896048 hasVolume "257" @default.
- W2014896048 isParatext "false" @default.
- W2014896048 isRetracted "false" @default.
- W2014896048 magId "2014896048" @default.
- W2014896048 workType "article" @default.