Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014900540> ?p ?o ?g. }
- W2014900540 abstract "Hepatitis B virus (HBV) is an important virus used in disinfection procedures for blood spillage. However, validation of HBV inactivation is difficult, since there are no feasible infectivity assays. In some countries, the duck HBV (DHBV) is recognized as a suitable model for testing antiviral activity of chemical biocides against HBV. Currently, DHBV-infected ducks are required for preparation of the test virus as well as eggs from DHBV-free flocks for testing DHBV infectivity. To improve the practicality of the system, we suggested to use commercially available embryonated duck eggs for preparation of DHBV-susceptible hepatocyte cultures and to exclude infected hepatocytes by pre-screening with qualitative detection of DHBV DNA using polymerase chain reaction (PCR). A standardized DHBV test virus was prepared from the DHBV DNA-transfected hepatoma cell line D2, which contained 10(11)DHBV DNA molecules per mL detected by light cycler real-time PCR. Infection of cell cultures was most efficient 4 days after plating. The best identification of infected cultures was possible 6 days after infection with immunofluorescence using an antiserum against DHBV surface antigen." @default.
- W2014900540 created "2016-06-24" @default.
- W2014900540 creator A5011928587 @default.
- W2014900540 creator A5017257878 @default.
- W2014900540 creator A5030711200 @default.
- W2014900540 creator A5031336069 @default.
- W2014900540 creator A5034989169 @default.
- W2014900540 creator A5043725161 @default.
- W2014900540 creator A5074207767 @default.
- W2014900540 creator A5082841663 @default.
- W2014900540 date "2005-11-01" @default.
- W2014900540 modified "2023-09-26" @default.
- W2014900540 title "Alternative methods for validation of cell culture infection with duck hepatitis B virus" @default.
- W2014900540 cites W12757830 @default.
- W2014900540 cites W1560334785 @default.
- W2014900540 cites W1778111758 @default.
- W2014900540 cites W1819564716 @default.
- W2014900540 cites W1845079004 @default.
- W2014900540 cites W1964572099 @default.
- W2014900540 cites W1964881340 @default.
- W2014900540 cites W1972805951 @default.
- W2014900540 cites W1976695098 @default.
- W2014900540 cites W2003989022 @default.
- W2014900540 cites W2006291551 @default.
- W2014900540 cites W2007902841 @default.
- W2014900540 cites W2008403400 @default.
- W2014900540 cites W2017275366 @default.
- W2014900540 cites W2061584067 @default.
- W2014900540 cites W2065129253 @default.
- W2014900540 cites W2070321506 @default.
- W2014900540 cites W2073831852 @default.
- W2014900540 cites W2078472691 @default.
- W2014900540 cites W2081326152 @default.
- W2014900540 cites W2082056709 @default.
- W2014900540 cites W2094090648 @default.
- W2014900540 cites W2103776714 @default.
- W2014900540 cites W2107432103 @default.
- W2014900540 cites W2122305232 @default.
- W2014900540 cites W2122440054 @default.
- W2014900540 cites W2124101714 @default.
- W2014900540 cites W2126864246 @default.
- W2014900540 cites W2134309152 @default.
- W2014900540 cites W2149945105 @default.
- W2014900540 cites W2153698842 @default.
- W2014900540 cites W2158857657 @default.
- W2014900540 cites W2278438969 @default.
- W2014900540 cites W2399700961 @default.
- W2014900540 cites W2474854370 @default.
- W2014900540 cites W597669211 @default.
- W2014900540 cites W765827980 @default.
- W2014900540 doi "https://doi.org/10.1016/j.jviromet.2005.05.025" @default.
- W2014900540 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16002155" @default.
- W2014900540 hasPublicationYear "2005" @default.
- W2014900540 type Work @default.
- W2014900540 sameAs 2014900540 @default.
- W2014900540 citedByCount "13" @default.
- W2014900540 countsByYear W20149005402012 @default.
- W2014900540 countsByYear W20149005402013 @default.
- W2014900540 countsByYear W20149005402014 @default.
- W2014900540 countsByYear W20149005402015 @default.
- W2014900540 crossrefType "journal-article" @default.
- W2014900540 hasAuthorship W2014900540A5011928587 @default.
- W2014900540 hasAuthorship W2014900540A5017257878 @default.
- W2014900540 hasAuthorship W2014900540A5030711200 @default.
- W2014900540 hasAuthorship W2014900540A5031336069 @default.
- W2014900540 hasAuthorship W2014900540A5034989169 @default.
- W2014900540 hasAuthorship W2014900540A5043725161 @default.
- W2014900540 hasAuthorship W2014900540A5074207767 @default.
- W2014900540 hasAuthorship W2014900540A5082841663 @default.
- W2014900540 hasConcept C104317684 @default.
- W2014900540 hasConcept C106358424 @default.
- W2014900540 hasConcept C129470801 @default.
- W2014900540 hasConcept C159047783 @default.
- W2014900540 hasConcept C159654299 @default.
- W2014900540 hasConcept C203014093 @default.
- W2014900540 hasConcept C2522874641 @default.
- W2014900540 hasConcept C2777666129 @default.
- W2014900540 hasConcept C2778236600 @default.
- W2014900540 hasConcept C2778600374 @default.
- W2014900540 hasConcept C2780593183 @default.
- W2014900540 hasConcept C2780617729 @default.
- W2014900540 hasConcept C49105822 @default.
- W2014900540 hasConcept C55493867 @default.
- W2014900540 hasConcept C86803240 @default.
- W2014900540 hasConceptScore W2014900540C104317684 @default.
- W2014900540 hasConceptScore W2014900540C106358424 @default.
- W2014900540 hasConceptScore W2014900540C129470801 @default.
- W2014900540 hasConceptScore W2014900540C159047783 @default.
- W2014900540 hasConceptScore W2014900540C159654299 @default.
- W2014900540 hasConceptScore W2014900540C203014093 @default.
- W2014900540 hasConceptScore W2014900540C2522874641 @default.
- W2014900540 hasConceptScore W2014900540C2777666129 @default.
- W2014900540 hasConceptScore W2014900540C2778236600 @default.
- W2014900540 hasConceptScore W2014900540C2778600374 @default.
- W2014900540 hasConceptScore W2014900540C2780593183 @default.
- W2014900540 hasConceptScore W2014900540C2780617729 @default.
- W2014900540 hasConceptScore W2014900540C49105822 @default.
- W2014900540 hasConceptScore W2014900540C55493867 @default.
- W2014900540 hasConceptScore W2014900540C86803240 @default.
- W2014900540 hasLocation W20149005401 @default.