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• W2014937751 abstract "Flow field-flow fractionation (FlFFF) with on-line UV/Visible diode array detector (DAD) and excitation emission matrix (EEM) fluorescence detector has been developed for the characterization of optical properties of aquatic dissolved organic matter (DOM) collected in the Otonabee River (Ontario, Canada) and Athabasca River (Alberta, Canada). The molecular weight (MW) distribution of DOM was estimated using a series of organic macromolecules ranging from 479 to 66,000 Da. Both the number-average (Mn) and weight-average (Mw) molecular weights of Suwannee River fulvic acid (SRFA) and Suwannee River humic acid (SRHA) determined using these macromolecular standards were comparable to those obtained using polystyrenesulfonate (PSS) standards, suggesting that organic macromolecules can be used to estimate MW of natural organic colloids. The MW of eight river DOM samples determined by this method was found to have an Mn range of 0.8–1.1 kDa, which agrees with available literature estimates. The FlFFF-DAD-EEM system provided insight into the MW components of river DOM including the optical properties by on-line absorbance and fluorescence measurement. A red-shift in emission and excitation wavelength maxima associated with lower spectral slope ratios (SR = S275–295:S350–400) was related to higher MW DOM. However, DOM of different origins at similar MW also showed significant difference in optical properties. A difference of 47 and 40 nm in excitation and emission peak C maxima was found. This supports the hypothesis that river DOM is not uniform in size and optical composition." @default.
• W2014937751 created "2016-06-24" @default.
• W2014937751 creator A5017401964 @default.
• W2014937751 creator A5068332037 @default.
• W2014937751 date "2011-07-01" @default.
• W2014937751 modified "2023-10-18" @default.
• W2014937751 title "Characterization of aquatic dissolved organic matter by asymmetrical flow field-flow fractionation coupled to UV–Visible diode array and excitation emission matrix fluorescence" @default.
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• W2014937751 doi "https://doi.org/10.1016/j.chroma.2010.12.038" @default.
• W2014937751 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21227433" @default.
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