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• W2014964025 endingPage "1319" @default.
• W2014964025 startingPage "1310" @default.
• W2014964025 abstract "The construction and performance under flow-injection conditions of an integrated amperometric biosensor for hydrogen peroxide is reported. The design of the bioelectrode is based on a mercaptopropionic acid (MPA) self-assembled monolayer (SAM) modified gold disk electrode on which horseradish peroxidase (HRP, 24.3 U) was immobilized by cross-linking with glutaraldehyde together with the mediator tetrathiafulvalene (TTF, 1 μmol), which was entrapped in the three-dimensional aggregate formed. The amperometric biosensor allows the obtention of reproducible flow injection amperometric responses at an applied potential of 0.00 V in 0.05 mol L−1 phosphate buffer, pH 7.0 (flow rate: 1.40 mL min−1, injection volume: 150 μL), with a range of linearity for hydrogen peroxide within the 2.0 × 10−7–1.0 × 10−4 mol L−1 concentration range (slope: (2.33 ± 0.02) × 10−2 A mol−1 L, r = 0.999). A detection limit of 6.9 × 10−8 mol L−1 was obtained together with a R.S.D. (n = 50) of 2.7% for a hydrogen peroxide concentration level of 5.0 × 10−5 mol L−1. The immobilization method showed a good reproducibility with a R.S.D. of 5.3% for five different electrodes. Moreover, the useful lifetime of one single biosensor was estimated in 13 days. The SAM-based biosensor was applied for the determination of hydrogen peroxide in rainwater and in a hair dye. The results obtained were validated by comparison with those obtained with a spectrophotometric reference method. In addition, the recovery of hydrogen peroxide in sterilised milk was tested." @default.
• W2014964025 created "2016-06-24" @default.
• W2014964025 creator A5014476523 @default.
• W2014964025 creator A5039506098 @default.
• W2014964025 creator A5082922049 @default.
• W2014964025 date "2005-06-15" @default.
• W2014964025 modified "2023-10-16" @default.
• W2014964025 title "A peroxidase-tetrathiafulvalene biosensor based on self-assembled monolayer modified Au electrodes for the flow-injection determination of hydrogen peroxide" @default.
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• W2014964025 doi "https://doi.org/10.1016/j.talanta.2005.01.048" @default.
• W2014964025 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18970123" @default.
• W2014964025 hasPublicationYear "2005" @default.
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