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- W2014992783 abstract "Abstract In yeast cells the dependence of amplitude and period of NADH oscillations on growth phase, starvation time and concentration of hexose fermented (D‐glucose of D‐fructose) has been studied. With both sugars sustained NADH oscillations could be induced during the first logarithmic growth phase only within a small range of starvation time (0.5 to 1.7 h). After transition to diauxic growth on ethanol, oscillations were obtained with D‐glucose from 0.5 to 6 h starvation time, whereas D‐fructose could induce oscillations only after 2 h of starvation. With D‐fructose instead of D‐glucose differences in period lengths of NADH oscillations resulted. These differences after D‐fructose feeding were always consistent and did not depend on the growth phase of the yeast culture. Amplitude and period lengths generally depended on starvation, resulting in stable period lengths after 3 h starvation whereas amplitudes stabilized after 5.3 h. With 0 to 50 mM hexose typical saturation kinetics resulted in amplitude and period of NADH oscillations. With D‐glucose (50 to 500 mM) amplitude and period stayed constant, whereas D‐fructose strongly quenched the oscillations in the same concentration range. It is concluded from these results that comparisons of NADH oscillations induced with D‐glucose or D‐fructose are only meaningful under constant conditions which are: harvesting of yeast culture at the end of the first logarithmic growdi phase, starvation for 5 hours, and 50 mM final hexose concentration. Under these conditions, it can be clearly shown that differences in amplitude and period of NADH oscillations are caused in D‐fructose metabolism." @default.
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- W2014992783 date "1979-04-01" @default.
- W2014992783 modified "2023-10-16" @default.
- W2014992783 title "Amplitude and period length of yeast NADH oscillations fermenting on different sugars in dependence of growth phase, starvation and hexose concentration" @default.
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- W2014992783 doi "https://doi.org/10.1080/09291017909359650" @default.
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