FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014995328> ?p ?o ?g. }

• W2014995328 endingPage "174" @default.
• W2014995328 startingPage "167" @default.
• W2014995328 abstract "In many streptococci, including Streptococcus mutans, genetic competence is regulated by a quorum sensing system mediated by a competence stimulating peptide (CSP) pheromone, encoded by the comC gene. In Streptococcus pneumoniae, a central component of this system is ComX, which acts as an alternative sigma factor to activate competence genes involved in DNA uptake and processing. The quorum sensing system responsible for genetic competence induction in S. mutans has been linked to biofilm formation and the acid tolerance response. To examine the response of comX to CSP in S. mutans, a transcriptional fusion of the comX promoter (pcomX) with lacZ was constructed to generate reporter vector pcomx::pALH122 (replicative vector) and transformed into S. mutans UA159 comC-, which is unable to produce endogenous CSP. CSP was added and pcomX::lacZ relative expression index (REI) examined, revealing a 2-fold increase in maximal beta-gal activity 5 and 10 min after CSP addition. The effect of endogenous CSP on pcomX::lacZ expression was also examined by measuring REI in cells grown as a biofilm; peak pcomX activity was observed at 3 h. To determine the temporal pattern of transformation frequency, pMA2, a Spr shuttle vector, was transformed into biofilm-grown cells, with maximal transformation frequency observed at 3 h. Confocal microscopy was performed to examine pcomX activity using a similarly constructed green fluorescent protein reporter vector, pcomX::gfp, in a 4-h biofilm, revealing active pcomX activity in high cell density areas within the biofilm population. These results demonstrated a positive correlation between pcomX activity, natural transformation and competence development in biofilms." @default.
• W2014995328 created "2016-06-24" @default.
• W2014995328 creator A5014704682 @default.
• W2014995328 creator A5043809137 @default.
• W2014995328 creator A5086497722 @default.
• W2014995328 date "2004-09-01" @default.
• W2014995328 modified "2023-09-24" @default.
• W2014995328 title "ComX activity of growing in biofilms" @default.
• W2014995328 cites W1747094814 @default.
• W2014995328 cites W1850703260 @default.
• W2014995328 cites W1970200721 @default.
• W2014995328 cites W1980689708 @default.
• W2014995328 cites W1987041144 @default.
• W2014995328 cites W1990379956 @default.
• W2014995328 cites W1995924937 @default.
• W2014995328 cites W2004056501 @default.
• W2014995328 cites W2018366249 @default.
• W2014995328 cites W2032579724 @default.
• W2014995328 cites W2043932025 @default.
• W2014995328 cites W2047929024 @default.
• W2014995328 cites W2061104342 @default.
• W2014995328 cites W2062221023 @default.
• W2014995328 cites W2066980596 @default.
• W2014995328 cites W2107757630 @default.
• W2014995328 cites W2108281648 @default.
• W2014995328 cites W2109112226 @default.
• W2014995328 cites W2111996478 @default.
• W2014995328 cites W2123886059 @default.
• W2014995328 cites W2134555745 @default.
• W2014995328 cites W2144440398 @default.
• W2014995328 cites W2146763898 @default.
• W2014995328 cites W2148337956 @default.
• W2014995328 cites W2149332722 @default.
• W2014995328 cites W2156846611 @default.
• W2014995328 cites W2158270839 @default.
• W2014995328 cites W2161978916 @default.
• W2014995328 cites W2163667594 @default.
• W2014995328 cites W3146406052 @default.
• W2014995328 doi "https://doi.org/10.1016/j.femsle.2004.07.032" @default.
• W2014995328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15336418" @default.
• W2014995328 hasPublicationYear "2004" @default.
• W2014995328 type Work @default.
• W2014995328 sameAs 2014995328 @default.
• W2014995328 citedByCount "27" @default.
• W2014995328 countsByYear W20149953282012 @default.
• W2014995328 countsByYear W20149953282013 @default.
• W2014995328 countsByYear W20149953282014 @default.
• W2014995328 countsByYear W20149953282015 @default.
• W2014995328 countsByYear W20149953282016 @default.
• W2014995328 countsByYear W20149953282017 @default.
• W2014995328 countsByYear W20149953282018 @default.
• W2014995328 countsByYear W20149953282019 @default.
• W2014995328 countsByYear W20149953282022 @default.
• W2014995328 countsByYear W20149953282023 @default.
• W2014995328 crossrefType "journal-article" @default.
• W2014995328 hasAuthorship W2014995328A5014704682 @default.
• W2014995328 hasAuthorship W2014995328A5043809137 @default.
• W2014995328 hasAuthorship W2014995328A5086497722 @default.
• W2014995328 hasConcept C104317684 @default.
• W2014995328 hasConcept C118687296 @default.
• W2014995328 hasConcept C142613039 @default.
• W2014995328 hasConcept C144024400 @default.
• W2014995328 hasConcept C145640855 @default.
• W2014995328 hasConcept C149923435 @default.
• W2014995328 hasConcept C150194340 @default.
• W2014995328 hasConcept C161733203 @default.
• W2014995328 hasConcept C204241405 @default.
• W2014995328 hasConcept C2778430296 @default.
• W2014995328 hasConcept C2908647359 @default.
• W2014995328 hasConcept C33161422 @default.
• W2014995328 hasConcept C40767141 @default.
• W2014995328 hasConcept C523546767 @default.
• W2014995328 hasConcept C54355233 @default.
• W2014995328 hasConcept C58123911 @default.
• W2014995328 hasConcept C86803240 @default.
• W2014995328 hasConcept C89423630 @default.
• W2014995328 hasConcept C92087593 @default.
• W2014995328 hasConcept C95444343 @default.
• W2014995328 hasConceptScore W2014995328C104317684 @default.
• W2014995328 hasConceptScore W2014995328C118687296 @default.
• W2014995328 hasConceptScore W2014995328C142613039 @default.
• W2014995328 hasConceptScore W2014995328C144024400 @default.
• W2014995328 hasConceptScore W2014995328C145640855 @default.
• W2014995328 hasConceptScore W2014995328C149923435 @default.
• W2014995328 hasConceptScore W2014995328C150194340 @default.
• W2014995328 hasConceptScore W2014995328C161733203 @default.
• W2014995328 hasConceptScore W2014995328C204241405 @default.
• W2014995328 hasConceptScore W2014995328C2778430296 @default.
• W2014995328 hasConceptScore W2014995328C2908647359 @default.
• W2014995328 hasConceptScore W2014995328C33161422 @default.
• W2014995328 hasConceptScore W2014995328C40767141 @default.
• W2014995328 hasConceptScore W2014995328C523546767 @default.
• W2014995328 hasConceptScore W2014995328C54355233 @default.
• W2014995328 hasConceptScore W2014995328C58123911 @default.
• W2014995328 hasConceptScore W2014995328C86803240 @default.
• W2014995328 hasConceptScore W2014995328C89423630 @default.
• W2014995328 hasConceptScore W2014995328C92087593 @default.
• W2014995328 hasConceptScore W2014995328C95444343 @default.

• next