Matches in SemOpenAlex for { <https://semopenalex.org/work/W2014998003> ?p ?o ?g. }
- W2014998003 endingPage "452" @default.
- W2014998003 startingPage "434" @default.
- W2014998003 abstract "Presynaptic active zones are essential structures for synaptic vesicle release, but the developmental regulation of their number and maintenance during aging at mammalian neuromuscular junctions (NMJs) remains unknown. Here, we analyzed the distribution of active zones in developing, mature, and aged mouse NMJs by immunohistochemical detection of the active zone-specific protein Bassoon. Bassoon is a cytosolic scaffolding protein essential for the active zone assembly in ribbon synapses and some brain synapses. Bassoon staining showed a punctate pattern in nerve terminals and axons at the nascent NMJ on embryonic days 16.5-18.5. Three-dimensional reconstruction of NMJs revealed that the majority of Bassoon puncta within an NMJ were attached to the presynaptic membrane from postnatal day 0 to adulthood, and colocalized with another active zone protein, Piccolo. During postnatal development, the number of Bassoon puncta increased as the size of the synapses increased. Importantly, the density of Bassoon puncta remained relatively constant from postnatal day 0 to 54 at 2.3 puncta/μm(2) , while the synapse size increased 3.3-fold. However, Bassoon puncta density and signal intensity were significantly attenuated at the NMJs of 27-month-old aged mice. These results suggest that synapses maintain the density of synaptic vesicle release sites while the synapse size changes, but this density becomes impaired during aging." @default.
- W2014998003 created "2016-06-24" @default.
- W2014998003 creator A5021271359 @default.
- W2014998003 creator A5026117179 @default.
- W2014998003 creator A5072575185 @default.
- W2014998003 date "2011-11-28" @default.
- W2014998003 modified "2023-10-02" @default.
- W2014998003 title "Active zone density is conserved during synaptic growth but impaired in aged mice" @default.
- W2014998003 cites W1512382989 @default.
- W2014998003 cites W1521104469 @default.
- W2014998003 cites W1590006710 @default.
- W2014998003 cites W1591639440 @default.
- W2014998003 cites W1592215258 @default.
- W2014998003 cites W1605791286 @default.
- W2014998003 cites W1664184517 @default.
- W2014998003 cites W1670422484 @default.
- W2014998003 cites W1782689717 @default.
- W2014998003 cites W1908945397 @default.
- W2014998003 cites W1930101093 @default.
- W2014998003 cites W1944633888 @default.
- W2014998003 cites W1963817246 @default.
- W2014998003 cites W1964902107 @default.
- W2014998003 cites W1965893090 @default.
- W2014998003 cites W1969183608 @default.
- W2014998003 cites W1969600766 @default.
- W2014998003 cites W1969901709 @default.
- W2014998003 cites W1970019903 @default.
- W2014998003 cites W1970539905 @default.
- W2014998003 cites W1971480441 @default.
- W2014998003 cites W1972530158 @default.
- W2014998003 cites W1972607225 @default.
- W2014998003 cites W1973841770 @default.
- W2014998003 cites W1974623748 @default.
- W2014998003 cites W1976438877 @default.
- W2014998003 cites W1981020765 @default.
- W2014998003 cites W1982026643 @default.
- W2014998003 cites W1982387497 @default.
- W2014998003 cites W1983169741 @default.
- W2014998003 cites W1989856883 @default.
- W2014998003 cites W1992858116 @default.
- W2014998003 cites W1994442356 @default.
- W2014998003 cites W1995098718 @default.
- W2014998003 cites W1997211923 @default.
- W2014998003 cites W1997668305 @default.
- W2014998003 cites W1998518207 @default.
- W2014998003 cites W2000898141 @default.
- W2014998003 cites W2001442825 @default.
- W2014998003 cites W2002352665 @default.
- W2014998003 cites W2007953565 @default.
- W2014998003 cites W2008776663 @default.
- W2014998003 cites W2009751169 @default.
- W2014998003 cites W2011819180 @default.
- W2014998003 cites W2013478045 @default.
- W2014998003 cites W2018341829 @default.
- W2014998003 cites W2018461674 @default.
- W2014998003 cites W2019116271 @default.
- W2014998003 cites W2024032585 @default.
- W2014998003 cites W2026036651 @default.
- W2014998003 cites W2026899274 @default.
- W2014998003 cites W2030094319 @default.
- W2014998003 cites W2037460206 @default.
- W2014998003 cites W2039467550 @default.
- W2014998003 cites W2039707783 @default.
- W2014998003 cites W2040478973 @default.
- W2014998003 cites W2041733763 @default.
- W2014998003 cites W2046854924 @default.
- W2014998003 cites W2050878615 @default.
- W2014998003 cites W2055198634 @default.
- W2014998003 cites W2056535632 @default.
- W2014998003 cites W2058057264 @default.
- W2014998003 cites W2058295628 @default.
- W2014998003 cites W2059854216 @default.
- W2014998003 cites W2065786892 @default.
- W2014998003 cites W2068244727 @default.
- W2014998003 cites W2071469863 @default.
- W2014998003 cites W2074378742 @default.
- W2014998003 cites W2080172302 @default.
- W2014998003 cites W2081967782 @default.
- W2014998003 cites W2093409301 @default.
- W2014998003 cites W2094279273 @default.
- W2014998003 cites W2094955667 @default.
- W2014998003 cites W2099348148 @default.
- W2014998003 cites W2102548112 @default.
- W2014998003 cites W2102734706 @default.
- W2014998003 cites W2104606868 @default.
- W2014998003 cites W2112522905 @default.
- W2014998003 cites W2113671810 @default.
- W2014998003 cites W2117911000 @default.
- W2014998003 cites W2121011070 @default.
- W2014998003 cites W2122067690 @default.
- W2014998003 cites W2127939995 @default.
- W2014998003 cites W2129350002 @default.
- W2014998003 cites W2134505400 @default.
- W2014998003 cites W2135414903 @default.
- W2014998003 cites W2137061790 @default.
- W2014998003 cites W2142080729 @default.
- W2014998003 cites W2143396171 @default.
- W2014998003 cites W2145437687 @default.