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- W2015000817 abstract "A method is described for the quantitative estimation of carbohydrates in glycolipids of tissues and body fluids. The glycolipids are extracted from pretreated tissues and, after removing interfering substances, are hydrolysed. The carbohydrate released may be estimated either by Somogyi's copper reagent or by the orcinol-sulphuric acid reaction. Using the latter reaction, the nature of the carbohydrate can be determined and the proportions of glucose and galactose estimated, if they occur together in the glycolipids. The average recovery of galactose in phrenosine is 93% with the orcinol-sulphuric acid reaction and 91% with the copper reagent of Somogyi. Factors responsible for this discrepancy as well as other steps in the procedure are investigated and discussed. Destruction of carbohydrate on acid treatment and incomplete hydrolysis of phrenosine were found to be the chief sources of error." @default.
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- W2015000817 date "1959-03-01" @default.
- W2015000817 modified "2023-09-23" @default.
- W2015000817 title "Estimation of carbohydrates of the glycolipids in tissues and body fluids" @default.
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- W2015000817 doi "https://doi.org/10.1016/0009-8981(59)90123-8" @default.
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