Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015011110> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2015011110 endingPage "S73" @default.
- W2015011110 startingPage "S73" @default.
- W2015011110 abstract "Perineuronal nets (PNNs) are specialized substructures of the neural extracellular matrix (ECM) which envelop the cell soma and proximal neurites of particular sets of neurons with apertures at sites of synaptic contact. Previous studies have shown that PNNs are enriched with chondroitin sulfate proteoglycans (CSPGs) and hyaluronan, however, a complete understanding of their precise molecular composition has been elusive. In addition, identifying which specific PNN components are critical to the formation of this structure has not been demonstrated. Previous work in our laboratory has demonstrated that the CSPG, aggrecan, is a key activity-dependent component of PNNs in vivo. In order to assess the contribution of aggrecan to PNN formation, we utilized cartilage matrix deficiency (cmd) mice, which lack aggrecan. Herein, we utilized an in vitro model, dissociated cortical culture, and an ex vivo model, organotypic slice culture, to specifically investigate the role aggrecan plays in PNN formation. Our work demonstrates that staining with the lectin, Wisteria floribunda agglutinin (WFA), considered a broad PNN marker, is eliminated in the absence of aggrecan, suggesting the loss of PNNs. However, in contrast, we found that the expression patterns of other PNN markers, including hyaluronan and proteoglycan link protein 1 (HAPLN1), tenascin-R, brevican, and hyaluronan are unaffected by the absence of aggrecan. Lastly, we determined that while all PNN components are bound to the surface in a hyaluronan-dependent manner, only HAPLN1 remains attached to the cell surface when neurons are treated with chondroitinase. These results suggest a different model for the molecular association of PNNs to the cell surface. Together our work has served to assess the contribution of aggrecan to PNN formation while providing key evidence concerning the molecular composition of PNNs in addition to determining how these components ultimately form PNNs." @default.
- W2015011110 created "2016-06-24" @default.
- W2015011110 creator A5025653335 @default.
- W2015011110 creator A5034652400 @default.
- W2015011110 creator A5083256966 @default.
- W2015011110 date "2009-01-01" @default.
- W2015011110 modified "2023-10-17" @default.
- W2015011110 title "Brevican organizes hyaluronan-binding extracellular assemblies distinctively at the large diameter nodes of Ranvier in the CNS" @default.
- W2015011110 doi "https://doi.org/10.1016/j.neures.2009.09.254" @default.
- W2015011110 hasPublicationYear "2009" @default.
- W2015011110 type Work @default.
- W2015011110 sameAs 2015011110 @default.
- W2015011110 citedByCount "0" @default.
- W2015011110 crossrefType "journal-article" @default.
- W2015011110 hasAuthorship W2015011110A5025653335 @default.
- W2015011110 hasAuthorship W2015011110A5034652400 @default.
- W2015011110 hasAuthorship W2015011110A5083256966 @default.
- W2015011110 hasConcept C12554922 @default.
- W2015011110 hasConcept C129987498 @default.
- W2015011110 hasConcept C142724271 @default.
- W2015011110 hasConcept C153074725 @default.
- W2015011110 hasConcept C185592680 @default.
- W2015011110 hasConcept C189165786 @default.
- W2015011110 hasConcept C197534660 @default.
- W2015011110 hasConcept C204787440 @default.
- W2015011110 hasConcept C2775895372 @default.
- W2015011110 hasConcept C2776164576 @default.
- W2015011110 hasConcept C2779335624 @default.
- W2015011110 hasConcept C2779553658 @default.
- W2015011110 hasConcept C2779794767 @default.
- W2015011110 hasConcept C3020332539 @default.
- W2015011110 hasConcept C55493867 @default.
- W2015011110 hasConcept C71924100 @default.
- W2015011110 hasConcept C86803240 @default.
- W2015011110 hasConcept C95444343 @default.
- W2015011110 hasConceptScore W2015011110C12554922 @default.
- W2015011110 hasConceptScore W2015011110C129987498 @default.
- W2015011110 hasConceptScore W2015011110C142724271 @default.
- W2015011110 hasConceptScore W2015011110C153074725 @default.
- W2015011110 hasConceptScore W2015011110C185592680 @default.
- W2015011110 hasConceptScore W2015011110C189165786 @default.
- W2015011110 hasConceptScore W2015011110C197534660 @default.
- W2015011110 hasConceptScore W2015011110C204787440 @default.
- W2015011110 hasConceptScore W2015011110C2775895372 @default.
- W2015011110 hasConceptScore W2015011110C2776164576 @default.
- W2015011110 hasConceptScore W2015011110C2779335624 @default.
- W2015011110 hasConceptScore W2015011110C2779553658 @default.
- W2015011110 hasConceptScore W2015011110C2779794767 @default.
- W2015011110 hasConceptScore W2015011110C3020332539 @default.
- W2015011110 hasConceptScore W2015011110C55493867 @default.
- W2015011110 hasConceptScore W2015011110C71924100 @default.
- W2015011110 hasConceptScore W2015011110C86803240 @default.
- W2015011110 hasConceptScore W2015011110C95444343 @default.
- W2015011110 hasLocation W20150111101 @default.
- W2015011110 hasOpenAccess W2015011110 @default.
- W2015011110 hasPrimaryLocation W20150111101 @default.
- W2015011110 hasRelatedWork W1890000587 @default.
- W2015011110 hasRelatedWork W1974815300 @default.
- W2015011110 hasRelatedWork W2015011110 @default.
- W2015011110 hasRelatedWork W2038737213 @default.
- W2015011110 hasRelatedWork W2064946371 @default.
- W2015011110 hasRelatedWork W2071526203 @default.
- W2015011110 hasRelatedWork W2072164463 @default.
- W2015011110 hasRelatedWork W2395135851 @default.
- W2015011110 hasRelatedWork W41427888 @default.
- W2015011110 hasRelatedWork W2478834987 @default.
- W2015011110 hasVolume "65" @default.
- W2015011110 isParatext "false" @default.
- W2015011110 isRetracted "false" @default.
- W2015011110 magId "2015011110" @default.
- W2015011110 workType "article" @default.