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- W2015013959 abstract "Cultured heart cells have been recently shown to be useful for analysing states of oxygen- and volume-restrictions, conditions that are known to simulate anoxia and ischemia at the cellular level. In the present study, we examined the ultrastructural damage caused to cultured neonatal rat heart cells when they were subjected to simimulated ischemia by volume restricted anoxia (‘ischemia’) in an in vitro system. Both thin-sectioning and freeze-fracturing electron microscopy revealed a mitochondrial reorganization after 30 min of ‘ischemia’, whereas multimellar structures could be detected inside the mitochondria after another 30 min. At this time-point, changes were also observed regarding the organization of the sarcolemma. In addition to a slight aggregation of the intramembranous particles (IMP's) we found an extensive extrusion of particle-free multimellar membrane-structures, possibly due to a loss of the sarcolemma/cytoskeleton-interaction. These morphological changes are comparable to those previously observed in in vivo and Langendorff studies and the results of the present study therefore underline the usefulness of this recently introduced model for ischemia." @default.
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- W2015013959 date "1974-12-01" @default.
- W2015013959 modified "2023-09-23" @default.
- W2015013959 title "Preservation of ultrastructure in freeze-fractured bacteria" @default.
- W2015013959 doi "https://doi.org/10.1016/0011-2240(74)90199-0" @default.
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