FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015090882> ?p ?o ?g. }

• W2015090882 abstract "Magnetotactic bacteria biomineralize ordered chains of uniform, membrane-bound magnetite or greigite nanocrystals that exhibit nearly perfect crystal structures and species-specific morphologies. Transmission electron microscopy (TEM) is a critical technique for providing information regarding the organization of cellular and magnetite structures in these microorganisms. However, conventional TEM can only be used to image air-dried or vitrified bacteria removed from their natural environment. Here we present a correlative scanning TEM (STEM) and fluorescence microscopy technique for imaging viable cells of Magnetospirillum magneticum strain AMB-1 in liquid using an in situ fluid cell TEM holder. Fluorescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell with STEM, followed by correlative fluorescence imaging to verify their membrane integrity. Notably, the post-STEM fluorescence imaging indicated that the bacterial cell wall membrane did not sustain radiation damage during STEM imaging at low electron dose conditions. We investigated the effects of radiation damage and sample preparation on the bacteria viability and found that approximately 50% of the bacterial membranes remained intact after an hour in the fluid cell, decreasing to ~30% after two hours. These results represent a first step toward in vivo studies of magnetite biomineralization in magnetotactic bacteria." @default.
• W2015090882 created "2016-06-24" @default.
• W2015090882 creator A5010988368 @default.
• W2015090882 creator A5011717241 @default.
• W2015090882 creator A5020127237 @default.
• W2015090882 creator A5032829801 @default.
• W2015090882 creator A5053735436 @default.
• W2015090882 creator A5055900838 @default.
• W2015090882 creator A5057097591 @default.
• W2015090882 creator A5066533134 @default.
• W2015090882 date "2014-10-31" @default.
• W2015090882 modified "2023-10-01" @default.
• W2015090882 title "Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging" @default.
• W2015090882 cites W1506656118 @default.
• W2015090882 cites W1600522855 @default.
• W2015090882 cites W1968148088 @default.
• W2015090882 cites W1970464799 @default.
• W2015090882 cites W1972391753 @default.
• W2015090882 cites W1979482476 @default.
• W2015090882 cites W1986765148 @default.
• W2015090882 cites W1991558541 @default.
• W2015090882 cites W2002099823 @default.
• W2015090882 cites W2002729596 @default.
• W2015090882 cites W2008573264 @default.
• W2015090882 cites W2015617882 @default.
• W2015090882 cites W2016445905 @default.
• W2015090882 cites W2019541463 @default.
• W2015090882 cites W2023417433 @default.
• W2015090882 cites W2026470835 @default.
• W2015090882 cites W2036999591 @default.
• W2015090882 cites W2041074303 @default.
• W2015090882 cites W2049053805 @default.
• W2015090882 cites W2049541663 @default.
• W2015090882 cites W2055294695 @default.
• W2015090882 cites W2058491936 @default.
• W2015090882 cites W2059704335 @default.
• W2015090882 cites W2061762695 @default.
• W2015090882 cites W2063882860 @default.
• W2015090882 cites W2071903506 @default.
• W2015090882 cites W2073758513 @default.
• W2015090882 cites W2077385785 @default.
• W2015090882 cites W2080853943 @default.
• W2015090882 cites W2081320341 @default.
• W2015090882 cites W2084374614 @default.
• W2015090882 cites W2093701175 @default.
• W2015090882 cites W2094549314 @default.
• W2015090882 cites W2100991647 @default.
• W2015090882 cites W2101826732 @default.
• W2015090882 cites W2104170190 @default.
• W2015090882 cites W2104835404 @default.
• W2015090882 cites W2106304089 @default.
• W2015090882 cites W2106393979 @default.
• W2015090882 cites W2109717868 @default.
• W2015090882 cites W2123350180 @default.
• W2015090882 cites W2123478027 @default.
• W2015090882 cites W2130892963 @default.
• W2015090882 cites W2131649583 @default.
• W2015090882 cites W2132555080 @default.
• W2015090882 cites W2135683001 @default.
• W2015090882 cites W2136073520 @default.
• W2015090882 cites W2142313173 @default.
• W2015090882 cites W2143864385 @default.
• W2015090882 cites W2144646955 @default.
• W2015090882 cites W2152024210 @default.
• W2015090882 cites W2155659696 @default.
• W2015090882 cites W2158511445 @default.
• W2015090882 cites W2163481756 @default.
• W2015090882 cites W2339307995 @default.
• W2015090882 cites W265705967 @default.
• W2015090882 cites W3102932905 @default.
• W2015090882 cites W53451662 @default.
• W2015090882 doi "https://doi.org/10.1038/srep06854" @default.
• W2015090882 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4215306" @default.
• W2015090882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25358460" @default.
• W2015090882 hasPublicationYear "2014" @default.
• W2015090882 type Work @default.
• W2015090882 sameAs 2015090882 @default.
• W2015090882 citedByCount "49" @default.
• W2015090882 countsByYear W20150908822015 @default.
• W2015090882 countsByYear W20150908822016 @default.
• W2015090882 countsByYear W20150908822017 @default.
• W2015090882 countsByYear W20150908822018 @default.
• W2015090882 countsByYear W20150908822019 @default.
• W2015090882 countsByYear W20150908822020 @default.
• W2015090882 countsByYear W20150908822021 @default.
• W2015090882 countsByYear W20150908822022 @default.
• W2015090882 crossrefType "journal-article" @default.
• W2015090882 hasAuthorship W2015090882A5010988368 @default.
• W2015090882 hasAuthorship W2015090882A5011717241 @default.
• W2015090882 hasAuthorship W2015090882A5020127237 @default.
• W2015090882 hasAuthorship W2015090882A5032829801 @default.
• W2015090882 hasAuthorship W2015090882A5053735436 @default.
• W2015090882 hasAuthorship W2015090882A5055900838 @default.
• W2015090882 hasAuthorship W2015090882A5057097591 @default.
• W2015090882 hasAuthorship W2015090882A5066533134 @default.
• W2015090882 hasBestOaLocation W20150908821 @default.
• W2015090882 hasConcept C120665830 @default.
• W2015090882 hasConcept C121332964 @default.
• W2015090882 hasConcept C12554922 @default.
• W2015090882 hasConcept C127413603 @default.

• next