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- W2015117531 abstract "This paper fabricated a cost-effective dsDNA-coupled plate (dcPlate) and applied it to measure the abundance and DNA-binding activity of a DNA-binding protein (DBP).The dcPlate was manufactured by covalently immobilizing an amino-modified oligonucleotide in wells of the plate coated with N-oxysuccinimide esters. The dcPlate was applied to measure the abundance of DNA-binding activity of a DBP in the same four steps, including protein incubation, primary antibody binding, enzyme-linked secondary antibody binding, and colorimetric development.The detections of three purified DBPs including NF-kappaB, AP1 and SP1, and HeLa cell nuclear extract and assays of DNA-binding activity of NF-kappaB p50 to five various DNA sequences demonstrated that dcPlate can be used to measure the abundance of DBPs quantitatively and assay DNA-binding activity of DBPs in high throughputs format.The homemade cost-effective dcPlate provides a simple and versatile platform for studying DBPs." @default.
- W2015117531 created "2016-06-24" @default.
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- W2015117531 date "2006-02-01" @default.
- W2015117531 modified "2023-09-26" @default.
- W2015117531 title "Assay of DNA-binding proteins with a dsDNA-coupled plate" @default.
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- W2015117531 doi "https://doi.org/10.1016/j.clinbiochem.2005.10.017" @default.
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