Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015130079> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2015130079 abstract "Partial metal depletion and 113Cd-NMR studies have suggested that the recombinant Cd-containing metallothionein of the sea urchin Strongylocentrotus purpuratus (Cd7-MTA) binds its metal ions in a four-metal (Cd4Cys11) and a three-metal (Cd3Cys9) cluster associated with the N-terminal and C-terminal halves of the protein, respectively [Wang, Y., Mackay, E.A., Zerbe, O., Hess, D., Hunziker, P.E., Vasak, M. & Kägi, J.H.R. (1995) Biochemistry 34,7460-7467]. This partitioning has now been confirmed by bisecting native Cd7-MTA with subtilisin into products bearing only a single metal-thiolate cluster. Their separation by reverse-phase HPLC and on-line electrospray mass spectrometry in combination with sequence analysis revealed selective cleavage of the protein into a set of N-terminal polypeptides containing 37-39 residues with four Cd ions and a set of C-terminal polypeptides containing 24 and 25 residues with three Cd ions. Thus, sea urchin MTA like its mammalian counterparts is made up of two separate cluster-harboring domains. The fragmentation pattern indicated that the sites of cleavage are located in the peptide loop interspaced between the first two metal-bound cysteine residues of the C-terminal domain. Accordingly, with cleavage, one of the putative nine thiolate ligands of the three-metal cluster was lost to the N-terminal fragment. The coordinational consequences of this repartition were reflected in massive chiroptical changes accompanying the cleavage process. While the liberated N-terminal domain retained the CD profile of the four-metal cluster in the parent protein and thereby indicated preservation of its structure, the CD features attributable to the intact three-metal cluster were largely lost on cleavage. The vanished features bear strong resemblance to the large biphasic ellipticity signal at 250 nm which dominates the CD spectrum of native Cd7-MTA, and allow us thus to attribute this signal to excitonic coupling interactions of Cd-thiolate chromophores in the three-metal cluster." @default.
- W2015130079 created "2016-06-24" @default.
- W2015130079 creator A5000083537 @default.
- W2015130079 creator A5006373917 @default.
- W2015130079 creator A5026142687 @default.
- W2015130079 creator A5044524448 @default.
- W2015130079 date "1996-11-01" @default.
- W2015130079 modified "2023-10-16" @default.
- W2015130079 title "Separation and Characterization of the Metal-Thiolate-Cluster Domains of Recombinant Sea Urchin Metallothionein" @default.
- W2015130079 cites W1485285854 @default.
- W2015130079 cites W1507671215 @default.
- W2015130079 cites W196233321 @default.
- W2015130079 cites W1964910363 @default.
- W2015130079 cites W1980387047 @default.
- W2015130079 cites W2008495072 @default.
- W2015130079 cites W2012570871 @default.
- W2015130079 cites W2038844755 @default.
- W2015130079 cites W2066228392 @default.
- W2015130079 cites W2068778863 @default.
- W2015130079 cites W2080916628 @default.
- W2015130079 cites W2086712433 @default.
- W2015130079 cites W2202406128 @default.
- W2015130079 cites W2333157284 @default.
- W2015130079 doi "https://doi.org/10.1111/j.1432-1033.1996.00835.x" @default.
- W2015130079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8944772" @default.
- W2015130079 hasPublicationYear "1996" @default.
- W2015130079 type Work @default.
- W2015130079 sameAs 2015130079 @default.
- W2015130079 citedByCount "20" @default.
- W2015130079 countsByYear W20151300792013 @default.
- W2015130079 countsByYear W20151300792014 @default.
- W2015130079 countsByYear W20151300792015 @default.
- W2015130079 crossrefType "journal-article" @default.
- W2015130079 hasAuthorship W2015130079A5000083537 @default.
- W2015130079 hasAuthorship W2015130079A5006373917 @default.
- W2015130079 hasAuthorship W2015130079A5026142687 @default.
- W2015130079 hasAuthorship W2015130079A5044524448 @default.
- W2015130079 hasConcept C104317684 @default.
- W2015130079 hasConcept C151730666 @default.
- W2015130079 hasConcept C167625842 @default.
- W2015130079 hasConcept C175156509 @default.
- W2015130079 hasConcept C178790620 @default.
- W2015130079 hasConcept C181199279 @default.
- W2015130079 hasConcept C185592680 @default.
- W2015130079 hasConcept C199164860 @default.
- W2015130079 hasConcept C20719415 @default.
- W2015130079 hasConcept C2776908312 @default.
- W2015130079 hasConcept C2777569064 @default.
- W2015130079 hasConcept C2779201268 @default.
- W2015130079 hasConcept C43369102 @default.
- W2015130079 hasConcept C535196362 @default.
- W2015130079 hasConcept C544153396 @default.
- W2015130079 hasConcept C55493867 @default.
- W2015130079 hasConcept C71240020 @default.
- W2015130079 hasConcept C86803240 @default.
- W2015130079 hasConceptScore W2015130079C104317684 @default.
- W2015130079 hasConceptScore W2015130079C151730666 @default.
- W2015130079 hasConceptScore W2015130079C167625842 @default.
- W2015130079 hasConceptScore W2015130079C175156509 @default.
- W2015130079 hasConceptScore W2015130079C178790620 @default.
- W2015130079 hasConceptScore W2015130079C181199279 @default.
- W2015130079 hasConceptScore W2015130079C185592680 @default.
- W2015130079 hasConceptScore W2015130079C199164860 @default.
- W2015130079 hasConceptScore W2015130079C20719415 @default.
- W2015130079 hasConceptScore W2015130079C2776908312 @default.
- W2015130079 hasConceptScore W2015130079C2777569064 @default.
- W2015130079 hasConceptScore W2015130079C2779201268 @default.
- W2015130079 hasConceptScore W2015130079C43369102 @default.
- W2015130079 hasConceptScore W2015130079C535196362 @default.
- W2015130079 hasConceptScore W2015130079C544153396 @default.
- W2015130079 hasConceptScore W2015130079C55493867 @default.
- W2015130079 hasConceptScore W2015130079C71240020 @default.
- W2015130079 hasConceptScore W2015130079C86803240 @default.
- W2015130079 hasLocation W20151300791 @default.
- W2015130079 hasLocation W20151300792 @default.
- W2015130079 hasOpenAccess W2015130079 @default.
- W2015130079 hasPrimaryLocation W20151300791 @default.
- W2015130079 hasRelatedWork W1970063456 @default.
- W2015130079 hasRelatedWork W1980133924 @default.
- W2015130079 hasRelatedWork W2015130079 @default.
- W2015130079 hasRelatedWork W2025004420 @default.
- W2015130079 hasRelatedWork W2028188779 @default.
- W2015130079 hasRelatedWork W2039915167 @default.
- W2015130079 hasRelatedWork W2041896721 @default.
- W2015130079 hasRelatedWork W2056704450 @default.
- W2015130079 hasRelatedWork W768049592 @default.
- W2015130079 hasRelatedWork W2513814133 @default.
- W2015130079 isParatext "false" @default.
- W2015130079 isRetracted "false" @default.
- W2015130079 magId "2015130079" @default.
- W2015130079 workType "article" @default.