FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015149742> ?p ?o ?g. }

• W2015149742 endingPage "55" @default.
• W2015149742 startingPage "43" @default.
• W2015149742 abstract "Summary— The object of the present paper is to complement the cytochemical detection of the polysacharides of the plant cell wall and of its precursors, taking benefit of two kinds of affinity methods: the enzyme‐gold technique and immunocytochemistry. Cellobiohydrolase (CBH 1, EC 3.2.1.91) was used to target native crystalline cellulose and two monoclonal antibodies, JIM 5 and JIM 7, were used to target homogalacturonan sequences with various degrees of esterification. Observations were performed at the light microscope level (UV epifluorescence, enzyme‐gold silver staining) and at the electron microscope level. Two types of biological specimens, both in steady state of growth, were chosen: in vitro cultures of melon cells (thin, unidirectional primary walls, loosely associated cells), and elongating zone of mung bean hypocotyl (thick walled and tightly associated cells). The following points were examined successively: the labelling at the histological level, the detection of cellulose and of polygalacturonan components in muro , the visualization of the emerging sites of the polymers along the endomembrane flow and their post‐synthetic modifications (crystallization and methylation respectively). JIM antibodies showed the early labelling of homogalacturonans on the bulging margins of the dictyosomes. The labelled vesicles appeared as sites of polymerization, cytoplasmic transport and beginning of molecular maturation with likely an early action of methyl transferases. The first labelling of cellulose occurred only on the outer face of the plasma‐membrane. Later on, CBH 1‐gold complexes remained distributed throughout the width of the growing wall, despite the surface expansion and the dispersion of the ordered framework. No significant change of the cellulose crystallinity was noticed. A co‐localization of polygalacturonan and cellulose markers was seen from the assembly to the deassembly of the cell wall. In complement, subtractive cytochemistry was performed using PATAg in association with an endopolygalacuronase to split the pectic chains or chelators (EDTA, EGTA, oxalate) to solubilize the calcium‐connected polyuronic acid chains. All the attacks exposed the individual microfibrils of the cellulose framework revealing uniformly the helicoidal organization and confirming that cellulose and polygalacturonans remain closely associated spatially during growth." @default.
• W2015149742 created "2016-06-24" @default.
• W2015149742 creator A5001710027 @default.
• W2015149742 creator A5017692238 @default.
• W2015149742 date "1991-01-01" @default.
• W2015149742 modified "2023-10-16" @default.
• W2015149742 title "Affinodetection of the sites of formation and of the further distribution of polygalacturonans and native cellulose in growing plant cells" @default.
• W2015149742 cites W1595832217 @default.
• W2015149742 cites W1912597336 @default.
• W2015149742 cites W1914893168 @default.
• W2015149742 cites W1968017414 @default.
• W2015149742 cites W1970122022 @default.
• W2015149742 cites W1997146296 @default.
• W2015149742 cites W2002198067 @default.
• W2015149742 cites W2003219510 @default.
• W2015149742 cites W2013788990 @default.
• W2015149742 cites W2015645237 @default.
• W2015149742 cites W2023272927 @default.
• W2015149742 cites W2027345965 @default.
• W2015149742 cites W2027416880 @default.
• W2015149742 cites W2030928457 @default.
• W2015149742 cites W2033584934 @default.
• W2015149742 cites W2036471469 @default.
• W2015149742 cites W2042325393 @default.
• W2015149742 cites W2042431767 @default.
• W2015149742 cites W2052452973 @default.
• W2015149742 cites W2067476028 @default.
• W2015149742 cites W2069065082 @default.
• W2015149742 cites W2077423871 @default.
• W2015149742 cites W2079341898 @default.
• W2015149742 cites W2090215320 @default.
• W2015149742 cites W2115658273 @default.
• W2015149742 cites W2156742794 @default.
• W2015149742 cites W2158658780 @default.
• W2015149742 cites W2168727725 @default.
• W2015149742 cites W2243259380 @default.
• W2015149742 cites W2315832387 @default.
• W2015149742 cites W2328965990 @default.
• W2015149742 cites W2419477884 @default.
• W2015149742 cites W2483379467 @default.
• W2015149742 cites W2498321153 @default.
• W2015149742 cites W947782702 @default.
• W2015149742 doi "https://doi.org/10.1016/0248-4900(91)90050-w" @default.
• W2015149742 hasPublicationYear "1991" @default.
• W2015149742 type Work @default.
• W2015149742 sameAs 2015149742 @default.
• W2015149742 citedByCount "39" @default.
• W2015149742 countsByYear W20151497422012 @default.
• W2015149742 countsByYear W20151497422014 @default.
• W2015149742 crossrefType "journal-article" @default.
• W2015149742 hasAuthorship W2015149742A5001710027 @default.
• W2015149742 hasAuthorship W2015149742A5017692238 @default.
• W2015149742 hasConcept C104317684 @default.
• W2015149742 hasConcept C121332964 @default.
• W2015149742 hasConcept C125235067 @default.
• W2015149742 hasConcept C12554922 @default.
• W2015149742 hasConcept C134018914 @default.
• W2015149742 hasConcept C156203801 @default.
• W2015149742 hasConcept C169274487 @default.
• W2015149742 hasConcept C190062978 @default.
• W2015149742 hasConcept C2779251873 @default.
• W2015149742 hasConcept C30030713 @default.
• W2015149742 hasConcept C35866371 @default.
• W2015149742 hasConcept C55493867 @default.
• W2015149742 hasConcept C59822182 @default.
• W2015149742 hasConcept C62520636 @default.
• W2015149742 hasConcept C7869135 @default.
• W2015149742 hasConcept C86803240 @default.
• W2015149742 hasConcept C87555872 @default.
• W2015149742 hasConcept C91881484 @default.
• W2015149742 hasConcept C95444343 @default.
• W2015149742 hasConceptScore W2015149742C104317684 @default.
• W2015149742 hasConceptScore W2015149742C121332964 @default.
• W2015149742 hasConceptScore W2015149742C125235067 @default.
• W2015149742 hasConceptScore W2015149742C12554922 @default.
• W2015149742 hasConceptScore W2015149742C134018914 @default.
• W2015149742 hasConceptScore W2015149742C156203801 @default.
• W2015149742 hasConceptScore W2015149742C169274487 @default.
• W2015149742 hasConceptScore W2015149742C190062978 @default.
• W2015149742 hasConceptScore W2015149742C2779251873 @default.
• W2015149742 hasConceptScore W2015149742C30030713 @default.
• W2015149742 hasConceptScore W2015149742C35866371 @default.
• W2015149742 hasConceptScore W2015149742C55493867 @default.
• W2015149742 hasConceptScore W2015149742C59822182 @default.
• W2015149742 hasConceptScore W2015149742C62520636 @default.
• W2015149742 hasConceptScore W2015149742C7869135 @default.
• W2015149742 hasConceptScore W2015149742C86803240 @default.
• W2015149742 hasConceptScore W2015149742C87555872 @default.
• W2015149742 hasConceptScore W2015149742C91881484 @default.
• W2015149742 hasConceptScore W2015149742C95444343 @default.
• W2015149742 hasIssue "1-2" @default.
• W2015149742 hasLocation W20151497421 @default.
• W2015149742 hasOpenAccess W2015149742 @default.
• W2015149742 hasPrimaryLocation W20151497421 @default.
• W2015149742 hasRelatedWork W114489217 @default.
• W2015149742 hasRelatedWork W2001465296 @default.
• W2015149742 hasRelatedWork W2030832342 @default.
• W2015149742 hasRelatedWork W2063722151 @default.

• next