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• W2015160249 startingPage "F1447" @default.
• W2015160249 abstract "Aquaporins and Rh proteins can function as gas (CO 2 and NH 3 ) channels. The present study explores the urea, H 2 O, CO 2 , and NH 3 permeability of the human urea transporter B (UT-B) (SLC14A1), expressed in Xenopus oocytes. We monitored urea uptake using [ 14 C]urea and measured osmotic water permeability ( P f ) using video microscopy. To obtain a semiquantitative measure of gas permeability, we used microelectrodes to record the maximum transient change in surface pH (ΔpH S ) caused by exposing oocytes to 5% CO 2 /33 mM HCO 3 − (pH S increase) or 0.5 mM NH 3 /NH 4 + (pH S decrease). UT-B expression increased oocyte permeability to urea by >20-fold, and P f by 8-fold vs. H 2 O-injected control oocytes. UT-B expression had no effect on the CO 2 -induced ΔpH S but doubled the NH 3 -induced ΔpH S . Phloretin reduced UT-B-dependent urea uptake ([Formula: see text]) by 45%, [Formula: see text] by 50%, and ([Formula: see text]) NH 3 by 70%. p-Chloromercuribenzene sulfonate reduced [Formula: see text] by 25%, [Formula: see text] by 30%, and ([Formula: see text]) NH 3 by 100%. Molecular dynamics (MD) simulations of membrane-embedded models of UT-B identified the monomeric UT-B pores as the main conduction pathway for both H 2 O and NH 3 and characterized the energetics associated with permeation of these species through the channel. Mutating each of two conserved threonines lining the monomeric urea pores reduced H 2 O and NH 3 permeability. Our data confirm that UT-B has significant H 2 O permeability and for the first time demonstrate significant NH 3 permeability. Thus the UTs become the third family of gas channels. Inhibitor and mutagenesis studies and results of MD simulations suggest that NH 3 and H 2 O pass through the three monomeric urea channels in UT-B." @default.
• W2015160249 created "2016-06-24" @default.
• W2015160249 creator A5031551936 @default.
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• W2015160249 date "2013-06-15" @default.
• W2015160249 modified "2023-09-23" @default.
• W2015160249 title "Movement of NH₃through the human urea transporter B: a new gas channel" @default.
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• W2015160249 doi "https://doi.org/10.1152/ajprenal.00609.2012" @default.
• W2015160249 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3680674" @default.
• W2015160249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23552862" @default.
• W2015160249 hasPublicationYear "2013" @default.
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