Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015164023> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2015164023 abstract "Purpose The purpose of this study was to analyze the phenotype of ENU induced Pxdn-/- eyes in mice(KTA48 mutants) and investigate the molecular mechanisms of peroxidasin in eye development and disease.Methods The eyes of the mutants were analyzed by morphologically and histologically. The molecular expression was measured by immunofluorescence, in situ hybridization and real time PCR.Results The genome-wide linkage analysis mapped the KTA48 mutation on chromosome 12; positional candidate gene analysis detected a mutation in the Pxdn gene (encoding peroxidasin) co-segregating with the mutation. The Pxdn-mRNA of the KTA48 mutants contains a T->A mutation at pos. 3816 (T3816A) creating a new Alw26I restriction site; the mutation converts the Cys at codon 1272 into a stop codon (Cys1272X).Whole mount immunostaining showed that peroxidasin is mainly expressed in the eye at E9.5. Immunostaining study showed peroxidasin is mainly express in the eye lids, developing cornea, lens, inner retina at embryonic stages. At E15.5, the phenotype of the homozygous mutant is most obvious but varies little, with its different tissues (especially anterior segment) severely impaired. At P11 Pxdn-/- mice showed further anomalies in addition to the phenotype in embryonic eyes, including congenital corneal opacity, congenital cataract and congenital glaucoma. Real-time PCR showed the expression of Pax6 is dramatically increased at E15.5 Pxdn-/- eyes compared to wild-type eyes, which was confirmed by immunofluorescence and in-situ hybridization.Conclusion Our findings demonstrate a requirement for peroxidasin in normal eye development, which may be through regulating Pax6 expression." @default.
- W2015164023 created "2016-06-24" @default.
- W2015164023 creator A5057469411 @default.
- W2015164023 creator A5064155313 @default.
- W2015164023 date "2012-08-06" @default.
- W2015164023 modified "2023-09-27" @default.
- W2015164023 title "A mutation in peroxidasin causes microphalmia and anterior segment dysgenesis in mice" @default.
- W2015164023 doi "https://doi.org/10.1111/j.1755-3768.2012.2464.x" @default.
- W2015164023 hasPublicationYear "2012" @default.
- W2015164023 type Work @default.
- W2015164023 sameAs 2015164023 @default.
- W2015164023 citedByCount "0" @default.
- W2015164023 crossrefType "journal-article" @default.
- W2015164023 hasAuthorship W2015164023A5057469411 @default.
- W2015164023 hasAuthorship W2015164023A5064155313 @default.
- W2015164023 hasConcept C104317684 @default.
- W2015164023 hasConcept C105580179 @default.
- W2015164023 hasConcept C127716648 @default.
- W2015164023 hasConcept C143065580 @default.
- W2015164023 hasConcept C153911025 @default.
- W2015164023 hasConcept C169760540 @default.
- W2015164023 hasConcept C203014093 @default.
- W2015164023 hasConcept C204232928 @default.
- W2015164023 hasConcept C2776882836 @default.
- W2015164023 hasConcept C2778085061 @default.
- W2015164023 hasConcept C2779812711 @default.
- W2015164023 hasConcept C4224716 @default.
- W2015164023 hasConcept C501734568 @default.
- W2015164023 hasConcept C51816534 @default.
- W2015164023 hasConcept C54355233 @default.
- W2015164023 hasConcept C69991583 @default.
- W2015164023 hasConcept C81439078 @default.
- W2015164023 hasConcept C86339819 @default.
- W2015164023 hasConcept C86803240 @default.
- W2015164023 hasConceptScore W2015164023C104317684 @default.
- W2015164023 hasConceptScore W2015164023C105580179 @default.
- W2015164023 hasConceptScore W2015164023C127716648 @default.
- W2015164023 hasConceptScore W2015164023C143065580 @default.
- W2015164023 hasConceptScore W2015164023C153911025 @default.
- W2015164023 hasConceptScore W2015164023C169760540 @default.
- W2015164023 hasConceptScore W2015164023C203014093 @default.
- W2015164023 hasConceptScore W2015164023C204232928 @default.
- W2015164023 hasConceptScore W2015164023C2776882836 @default.
- W2015164023 hasConceptScore W2015164023C2778085061 @default.
- W2015164023 hasConceptScore W2015164023C2779812711 @default.
- W2015164023 hasConceptScore W2015164023C4224716 @default.
- W2015164023 hasConceptScore W2015164023C501734568 @default.
- W2015164023 hasConceptScore W2015164023C51816534 @default.
- W2015164023 hasConceptScore W2015164023C54355233 @default.
- W2015164023 hasConceptScore W2015164023C69991583 @default.
- W2015164023 hasConceptScore W2015164023C81439078 @default.
- W2015164023 hasConceptScore W2015164023C86339819 @default.
- W2015164023 hasConceptScore W2015164023C86803240 @default.
- W2015164023 hasLocation W20151640231 @default.
- W2015164023 hasOpenAccess W2015164023 @default.
- W2015164023 hasPrimaryLocation W20151640231 @default.
- W2015164023 hasRelatedWork W161705314 @default.
- W2015164023 hasRelatedWork W1979831998 @default.
- W2015164023 hasRelatedWork W1992684616 @default.
- W2015164023 hasRelatedWork W2031094162 @default.
- W2015164023 hasRelatedWork W2058325441 @default.
- W2015164023 hasRelatedWork W2065022459 @default.
- W2015164023 hasRelatedWork W2093717293 @default.
- W2015164023 hasRelatedWork W2094214238 @default.
- W2015164023 hasRelatedWork W2094999649 @default.
- W2015164023 hasRelatedWork W2100052572 @default.
- W2015164023 hasRelatedWork W2127482249 @default.
- W2015164023 hasRelatedWork W2133218249 @default.
- W2015164023 hasRelatedWork W2163357493 @default.
- W2015164023 hasRelatedWork W2164628276 @default.
- W2015164023 hasRelatedWork W2592461139 @default.
- W2015164023 hasRelatedWork W2772238185 @default.
- W2015164023 hasRelatedWork W2937146189 @default.
- W2015164023 hasRelatedWork W2994232137 @default.
- W2015164023 hasRelatedWork W40215388 @default.
- W2015164023 hasRelatedWork W2604942128 @default.
- W2015164023 isParatext "false" @default.
- W2015164023 isRetracted "false" @default.
- W2015164023 magId "2015164023" @default.
- W2015164023 workType "article" @default.