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• W2015166075 abstract "Metabolic labeling of rodent proteins with ¹⁵N, a heavy stable isotope of nitrogen, provides an efficient way for relative quantitation of differentially expressed proteins. Here we describe a protocol for metabolic labeling of rats with an ¹⁵N-enriched spirulina diet. As a case study, we also demonstrate the application of ¹⁵N-enriched tissue as a common internal standard in quantitative analysis of differentially expressed proteins in neurodevelopment in rats at two different time points, postnatal day 1 and 45. We briefly discuss the bioinformatics tools, ProLucid and Census, which can easily be used in a sequential manner to identify and quantitate relative protein levels on a proteomic scale." @default.
• W2015166075 created "2016-06-24" @default.
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• W2015166075 date "2013-06-01" @default.
• W2015166075 modified "2023-10-13" @default.
• W2015166075 title "Stable isotope labeling of mammals (SILAM) for in vivo quantitative proteomic analysis" @default.
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• W2015166075 doi "https://doi.org/10.1016/j.ymeth.2013.03.008" @default.
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