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• W2015173791 abstract "Food falsification has direct impact on public health, religious faith, fair-trades and wildlife. For the first time, here we described a multiplex polymerase chain reaction assay for the accurate identification of five meat species forbidden in Islamic foods in a single assay platform. Five pairs of species-specific primers were designed targeting mitochondrial ND5, ATPase 6, and cytochrome b genes to amplify 172, 163, 141, 129 and 108 bp DNA fragments from cat, dog, pig, monkey and rat meats, respectively. All PCR products were identified in gel-images and electrochromatograms obtained from Experion Bioanalyzer. Species-specificity checking against 15 important meat and fish and 5 plant species detected no cross-species amplification. Screening of target species in model and commercial meatballs reflected its application to detect target species in process foods. The assay was tested to detect 0.01–0.02 ng DNA under raw states and 1% suspected meats in meatball formulation." @default.
• W2015173791 created "2016-06-24" @default.
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• W2015173791 date "2015-06-01" @default.
• W2015173791 modified "2023-10-12" @default.
• W2015173791 title "Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods" @default.
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• W2015173791 doi "https://doi.org/10.1016/j.foodchem.2014.12.098" @default.
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