FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015184543> ?p ?o ?g. }

• W2015184543 endingPage "512" @default.
• W2015184543 startingPage "497" @default.
• W2015184543 abstract "The FF domain from the human protein HYPA/FBP11 folds via a low-energy on-pathway intermediate (I). Elucidation of the structure of such folding intermediates and denatured states under conditions that favour folding are difficult tasks. Here, we investigated the millisecond time-scale equilibrium folding transition of the 71-residue four-helix bundle wild-type protein by (15)N, (13)C(alpha) and methyl(13)C Carr-Purcell-Meiboom-Gill (CPMG) NMR relaxation dispersion experiments and by (1)H/(2)H-exchange measurements. The relaxation data for the wild-type protein fitted a simple two-site exchange process between the folded state (F) and I. Destabilization of F in mutants A17G and Q19G allowed the detection of the unfolded state U by (15)N CPMG relaxation dispersion. The dispersion data for these mutants fitted a three-site exchange scheme, U<-->I<-->F, with I populated higher than U. The kinetics and thermodynamics of the folding reaction were obtained via temperature and urea-dependent relaxation dispersion experiments, along with structural information on I from backbone (15)N, (13)C(alpha) and side-chain methyl (13)C chemical shifts, with further information from protection factors for the backbone amide groups from (1)H/(2)H-exchange. Notably, helices H1-H3 are at least partially formed in I, while helix H4 is largely disordered. Chemical shift differences for the methyl (13)C nuclei suggest a paucity of stable, native-like hydrophobic interactions in I. These data are consistent with Phi-analysis of the rate-limiting transition state between I and F. The combination of relaxation dispersion and Phi data can elucidate whole experimental folding pathways." @default.
• W2015184543 created "2016-06-24" @default.
• W2015184543 creator A5008065534 @default.
• W2015184543 creator A5018102939 @default.
• W2015184543 creator A5020050875 @default.
• W2015184543 creator A5068711489 @default.
• W2015184543 creator A5086370446 @default.
• W2015184543 date "2007-09-01" @default.
• W2015184543 modified "2023-09-26" @default.
• W2015184543 title "The Folding Pathway of an FF domain: Characterization of an On-pathway Intermediate State Under Folding Conditions by 15N, 13Cα and 13C-methyl Relaxation Dispersion and 1H/2H-exchange NMR Spectroscopy" @default.
• W2015184543 cites W1588812136 @default.
• W2015184543 cites W1593625064 @default.
• W2015184543 cites W1593987163 @default.
• W2015184543 cites W1965676565 @default.
• W2015184543 cites W1967702166 @default.
• W2015184543 cites W1968640720 @default.
• W2015184543 cites W1969103850 @default.
• W2015184543 cites W1973060931 @default.
• W2015184543 cites W1973897761 @default.
• W2015184543 cites W1976025783 @default.
• W2015184543 cites W1976270316 @default.
• W2015184543 cites W1977179882 @default.
• W2015184543 cites W1978017493 @default.
• W2015184543 cites W1978314322 @default.
• W2015184543 cites W1981159786 @default.
• W2015184543 cites W1981448638 @default.
• W2015184543 cites W1982013103 @default.
• W2015184543 cites W1984830795 @default.
• W2015184543 cites W1985960343 @default.
• W2015184543 cites W1987528091 @default.
• W2015184543 cites W1988058873 @default.
• W2015184543 cites W1990126142 @default.
• W2015184543 cites W1990721255 @default.
• W2015184543 cites W1991591212 @default.
• W2015184543 cites W1994681621 @default.
• W2015184543 cites W1994939140 @default.
• W2015184543 cites W1995216190 @default.
• W2015184543 cites W1996855174 @default.
• W2015184543 cites W2000513450 @default.
• W2015184543 cites W2003119577 @default.
• W2015184543 cites W2007221727 @default.
• W2015184543 cites W2007832693 @default.
• W2015184543 cites W2007886752 @default.
• W2015184543 cites W2008440654 @default.
• W2015184543 cites W2020695579 @default.
• W2015184543 cites W2025246413 @default.
• W2015184543 cites W2027211622 @default.
• W2015184543 cites W2029871341 @default.
• W2015184543 cites W2031292508 @default.
• W2015184543 cites W2031628248 @default.
• W2015184543 cites W2032435646 @default.
• W2015184543 cites W2033762168 @default.
• W2015184543 cites W2045066191 @default.
• W2015184543 cites W2046452853 @default.
• W2015184543 cites W2048338648 @default.
• W2015184543 cites W2048740018 @default.
• W2015184543 cites W2053799953 @default.
• W2015184543 cites W2054191418 @default.
• W2015184543 cites W2054639560 @default.
• W2015184543 cites W2055557864 @default.
• W2015184543 cites W2059214571 @default.
• W2015184543 cites W2064100411 @default.
• W2015184543 cites W2071556057 @default.
• W2015184543 cites W2073662901 @default.
• W2015184543 cites W2078070626 @default.
• W2015184543 cites W2082176639 @default.
• W2015184543 cites W2088397672 @default.
• W2015184543 cites W2092356013 @default.
• W2015184543 cites W2114320763 @default.
• W2015184543 cites W2124649680 @default.
• W2015184543 cites W2131625617 @default.
• W2015184543 cites W2140213897 @default.
• W2015184543 cites W2141098684 @default.
• W2015184543 cites W2148521301 @default.
• W2015184543 cites W2152601396 @default.
• W2015184543 cites W2161859154 @default.
• W2015184543 cites W240415867 @default.
• W2015184543 cites W275584459 @default.
• W2015184543 cites W4242009576 @default.
• W2015184543 doi "https://doi.org/10.1016/j.jmb.2007.06.012" @default.
• W2015184543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17689561" @default.
• W2015184543 hasPublicationYear "2007" @default.
• W2015184543 type Work @default.
• W2015184543 sameAs 2015184543 @default.
• W2015184543 citedByCount "58" @default.
• W2015184543 countsByYear W20151845432012 @default.
• W2015184543 countsByYear W20151845432013 @default.
• W2015184543 countsByYear W20151845432014 @default.
• W2015184543 countsByYear W20151845432015 @default.
• W2015184543 countsByYear W20151845432016 @default.
• W2015184543 countsByYear W20151845432017 @default.
• W2015184543 countsByYear W20151845432018 @default.
• W2015184543 countsByYear W20151845432019 @default.
• W2015184543 countsByYear W20151845432020 @default.
• W2015184543 countsByYear W20151845432021 @default.
• W2015184543 countsByYear W20151845432022 @default.
• W2015184543 countsByYear W20151845432023 @default.
• W2015184543 crossrefType "journal-article" @default.

• next