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- W2015199411 abstract "The 3′ terminus of the (-) RNA strand in the replicative forms of several (+)-stranded RNA viruses possesses an unpaired guanosine with unknown function. This unpaired guanosine is also found at the 3′ terminus of the (-) strand in the double-stranded form of two cucumoviral satellite RNAs. Using a cucumber mosaic virus (CMV) RNA-dependent RNA polymerase capable of replicating the satellite RNA in vitro, the 3′-terminal guanosine of the satellite (-) strand was shown to be an absolute requirement for satellite (+) strand synthesis. If genomic RNA synthesis of CMV and other members of the alphavirus-like superfamily that produce (-) strands terminating in an unpaired 3′ guanosine follows a similar strategy, the work reported here would represent the first experimental support for the notion of 3′-terminal guanosine functioning as an essential recognition signal for viral replicases, enabling (+) strand RNA synthesis to be initiated internally from a (-) strand template." @default.
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- W2015199411 date "1994-05-01" @default.
- W2015199411 modified "2023-10-17" @default.
- W2015199411 title "Requirement of 3′-Terminal Guanosine in (-)-Stranded RNA for in vitro Replication of Cucumber Mosaic Virus Satellite RNA by Viral RNA-dependent RNA Polymerase" @default.
- W2015199411 doi "https://doi.org/10.1006/jmbi.1994.1326" @default.
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