Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015216916> ?p ?o ?g. }
- W2015216916 endingPage "425" @default.
- W2015216916 startingPage "416" @default.
- W2015216916 abstract "The main source of mitochondrial DNA (mtDNA) damage is reactive oxygen species (ROS) generated during normal cellular metabolism. The main mtDNA lesions generated by ROS are base modifications, such as the ubiquitous 8-oxoguanine (8-oxoG) lesion; however, base loss and strand breaks may also occur. Many human diseases are associated with mtDNA mutations and thus maintaining mtDNA integrity is critical. All of these lesions are repaired primarily by the base excision repair (BER) pathway. It is now known that mammalian mitochondria have BER, which, similarly to nuclear BER, is catalyzed by DNA glycosylases, AP endonuclease, DNA polymerase (POLγ in mitochondria) and DNA ligase. This article outlines procedures for measuring oxidative damage formation and BER in mitochondria, including isolation of mitochondria from tissues and cells, protocols for measuring BER enzyme activities, gene-specific repair assays, chromatographic techniques as well as current optimizations for detecting 8-oxoG lesions in cells by immunofluorescence. Throughout the assay descriptions we will include methodological considerations that may help optimize the assays in terms of resolution and repeatability." @default.
- W2015216916 created "2016-06-24" @default.
- W2015216916 creator A5004632090 @default.
- W2015216916 creator A5051173396 @default.
- W2015216916 creator A5069851925 @default.
- W2015216916 creator A5089917586 @default.
- W2015216916 date "2010-08-01" @default.
- W2015216916 modified "2023-09-27" @default.
- W2015216916 title "Mitochondrial base excision repair assays" @default.
- W2015216916 cites W1490730914 @default.
- W2015216916 cites W1497265707 @default.
- W2015216916 cites W1532431885 @default.
- W2015216916 cites W1547707281 @default.
- W2015216916 cites W1552366746 @default.
- W2015216916 cites W1552620600 @default.
- W2015216916 cites W1559227319 @default.
- W2015216916 cites W1602460334 @default.
- W2015216916 cites W1751954544 @default.
- W2015216916 cites W1802480460 @default.
- W2015216916 cites W1926649163 @default.
- W2015216916 cites W19371791 @default.
- W2015216916 cites W1960438546 @default.
- W2015216916 cites W1966571906 @default.
- W2015216916 cites W1966799056 @default.
- W2015216916 cites W1968499584 @default.
- W2015216916 cites W1970590066 @default.
- W2015216916 cites W1971160716 @default.
- W2015216916 cites W1971602039 @default.
- W2015216916 cites W1972802911 @default.
- W2015216916 cites W1974259669 @default.
- W2015216916 cites W1979222777 @default.
- W2015216916 cites W1979285704 @default.
- W2015216916 cites W1979516784 @default.
- W2015216916 cites W1979610420 @default.
- W2015216916 cites W1985133999 @default.
- W2015216916 cites W1986636389 @default.
- W2015216916 cites W1987599974 @default.
- W2015216916 cites W1990625194 @default.
- W2015216916 cites W1992209005 @default.
- W2015216916 cites W1994496497 @default.
- W2015216916 cites W1995202749 @default.
- W2015216916 cites W1995484312 @default.
- W2015216916 cites W1996809120 @default.
- W2015216916 cites W1999125434 @default.
- W2015216916 cites W2005356916 @default.
- W2015216916 cites W2006391458 @default.
- W2015216916 cites W2014903992 @default.
- W2015216916 cites W2014972109 @default.
- W2015216916 cites W2020383780 @default.
- W2015216916 cites W2023832320 @default.
- W2015216916 cites W2033085735 @default.
- W2015216916 cites W2034418364 @default.
- W2015216916 cites W2036590181 @default.
- W2015216916 cites W2037211079 @default.
- W2015216916 cites W2037349364 @default.
- W2015216916 cites W2037542399 @default.
- W2015216916 cites W2037603814 @default.
- W2015216916 cites W2039850925 @default.
- W2015216916 cites W2048411197 @default.
- W2015216916 cites W2049217916 @default.
- W2015216916 cites W2053619432 @default.
- W2015216916 cites W2067275981 @default.
- W2015216916 cites W2068050243 @default.
- W2015216916 cites W2076554838 @default.
- W2015216916 cites W2080272457 @default.
- W2015216916 cites W2082144941 @default.
- W2015216916 cites W2086339686 @default.
- W2015216916 cites W2087597149 @default.
- W2015216916 cites W2088715137 @default.
- W2015216916 cites W2089038479 @default.
- W2015216916 cites W2089257213 @default.
- W2015216916 cites W2092629964 @default.
- W2015216916 cites W2092845419 @default.
- W2015216916 cites W2093271396 @default.
- W2015216916 cites W2094503136 @default.
- W2015216916 cites W2105374260 @default.
- W2015216916 cites W2107043033 @default.
- W2015216916 cites W2108747861 @default.
- W2015216916 cites W2109099295 @default.
- W2015216916 cites W2109291482 @default.
- W2015216916 cites W2109323414 @default.
- W2015216916 cites W2115638153 @default.
- W2015216916 cites W2115854588 @default.
- W2015216916 cites W2117795149 @default.
- W2015216916 cites W2121454542 @default.
- W2015216916 cites W2121668789 @default.
- W2015216916 cites W2122677203 @default.
- W2015216916 cites W2122785328 @default.
- W2015216916 cites W2134428957 @default.
- W2015216916 cites W2136995982 @default.
- W2015216916 cites W2137854060 @default.
- W2015216916 cites W2139020323 @default.
- W2015216916 cites W2155197966 @default.
- W2015216916 cites W2155666009 @default.
- W2015216916 cites W2161085054 @default.
- W2015216916 cites W2165604195 @default.
- W2015216916 cites W2167525878 @default.
- W2015216916 cites W2200460224 @default.