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- W2015220614 abstract "Abstract DNA replication in Escherichia coli was followed using analytical composite agarose-acrylamide gel electrophoresis of native and denatured DNA samples from cells that had been pulse-labeled in vivo . The results obtained with samples of native DNA, combined with the different sensitivities of the various electrophoretic fractions of native DNA to enzymatic attack, led us to conclude that a significant amount of single-strandedness exists in the region of the replication site. Data obtained from kinetic analyses of the labeling of both the high molecular weight DNA and the fragments formed on denaturation of native DNA samples were consistent with the Okazaki model (Okazaki et al. , 1968 a,b ) of DNA replication. Furthermore, the data indicated that eight to ten precursor fragments are present per fork during bacterial growth at both 20 and 37 °C, under the conditions used in this study." @default.
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- W2015220614 date "1974-04-01" @default.
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- W2015220614 title "DNA replication in Escherichia coli: Physical and kinetic studies of the replication point" @default.
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- W2015220614 doi "https://doi.org/10.1016/0022-2836(74)90585-3" @default.
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