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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015222457> ?p ?o ?g. }

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• W2015222457 abstract "Lentiviral expression vectors encoding short hairpin RNA (shRNA) are widely used for RNAi-based gene silencing in mammalian cells. However, current methods for the construction of shRNA expression vectors require multiple steps, which are expensive, time-consuming, and error-prone. Here, we developed a single-step mixing cloning method for the generation of lentiviral shRNA expression vectors. With this method, a pair of short oligonucleotides (∼50 nt) is required and a lentiviral shRNA vector can be constructed with only one step. This method has been used to construct 30 lentiviral shRNA expression vectors successfully." @default.
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• W2015222457 date "2013-07-01" @default.
• W2015222457 modified "2023-10-17" @default.
• W2015222457 title "A single-step mixing cloning method for assembly of lentiviral short hairpin RNA expression vectors for gene silencing" @default.
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• W2015222457 doi "https://doi.org/10.1016/j.ab.2013.03.011" @default.
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