Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015243129> ?p ?o ?g. }
- W2015243129 endingPage "2268" @default.
- W2015243129 startingPage "2259" @default.
- W2015243129 abstract "RNA polymerase III (pol III) transcripts are abundant in all cells. Therefore, pol III promoters may be ideal for expressing high levels of exogenous RNAs, such as antisense RNAs, decoy RNAs and ribozymes, in many different cell types. We have improved accumulation of recombinant RNAs expressed from a human met1 tRNA-derived pol III promoter >100-fold by modifying the 3′ terminus of the transcripts to hybridize to the 5′ terminus. This terminal duplex Includes the 8 nt leader sequence present in the primary wild-type met) tRNA transcript that is normally removed during processing to the mature tRNA. Expression of an anti-HIV ribozyme was analyzed in cells stably transduced with retroviral vectors encoding pol III transcription units containing this modification. High accumulation of recombinant pol III ribozyme transcripts was observed in all cell lines tested. Due to the enhanced transcript accumulation, ribozyme cleavage activity was readily detectable in total RNA extracted from stably transduced human T cell lines. One pol III transcription unit, termed ‘TRZ’, was optimized further for ribozyme cleavage activity. The improved pol III transcription units reported here may be useful for expressing a variety of functional and therapeutic RNAs." @default.
- W2015243129 created "2016-06-24" @default.
- W2015243129 creator A5003137417 @default.
- W2015243129 creator A5018318119 @default.
- W2015243129 creator A5020886114 @default.
- W2015243129 creator A5028413988 @default.
- W2015243129 creator A5029984118 @default.
- W2015243129 creator A5031918109 @default.
- W2015243129 creator A5065020856 @default.
- W2015243129 creator A5081343739 @default.
- W2015243129 date "1995-01-01" @default.
- W2015243129 modified "2023-09-23" @default.
- W2015243129 title "Improved accumulation and activity of ribozymes expressed from a tRNA-based RNA polymerase III promoter" @default.
- W2015243129 cites W1524880457 @default.
- W2015243129 cites W1530482967 @default.
- W2015243129 cites W1654780472 @default.
- W2015243129 cites W1676925074 @default.
- W2015243129 cites W1800005238 @default.
- W2015243129 cites W1876905084 @default.
- W2015243129 cites W1964858690 @default.
- W2015243129 cites W1973705988 @default.
- W2015243129 cites W1985881197 @default.
- W2015243129 cites W1995403172 @default.
- W2015243129 cites W1998281796 @default.
- W2015243129 cites W1999391211 @default.
- W2015243129 cites W2004537665 @default.
- W2015243129 cites W2015326137 @default.
- W2015243129 cites W2017313617 @default.
- W2015243129 cites W2024167450 @default.
- W2015243129 cites W2024568805 @default.
- W2015243129 cites W2026102468 @default.
- W2015243129 cites W2031092694 @default.
- W2015243129 cites W2039977144 @default.
- W2015243129 cites W2067653729 @default.
- W2015243129 cites W2079321730 @default.
- W2015243129 cites W2084208256 @default.
- W2015243129 cites W2088735794 @default.
- W2015243129 cites W2108276123 @default.
- W2015243129 cites W2115066994 @default.
- W2015243129 cites W2124860623 @default.
- W2015243129 cites W2134812217 @default.
- W2015243129 cites W2156102046 @default.
- W2015243129 cites W2158626412 @default.
- W2015243129 cites W2234928500 @default.
- W2015243129 cites W2404483442 @default.
- W2015243129 cites W2461163283 @default.
- W2015243129 doi "https://doi.org/10.1093/nar/23.12.2259" @default.
- W2015243129 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/307016" @default.
- W2015243129 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7610054" @default.
- W2015243129 hasPublicationYear "1995" @default.
- W2015243129 type Work @default.
- W2015243129 sameAs 2015243129 @default.
- W2015243129 citedByCount "74" @default.
- W2015243129 countsByYear W20152431292012 @default.
- W2015243129 countsByYear W20152431292016 @default.
- W2015243129 countsByYear W20152431292018 @default.
- W2015243129 crossrefType "journal-article" @default.
- W2015243129 hasAuthorship W2015243129A5003137417 @default.
- W2015243129 hasAuthorship W2015243129A5018318119 @default.
- W2015243129 hasAuthorship W2015243129A5020886114 @default.
- W2015243129 hasAuthorship W2015243129A5028413988 @default.
- W2015243129 hasAuthorship W2015243129A5029984118 @default.
- W2015243129 hasAuthorship W2015243129A5031918109 @default.
- W2015243129 hasAuthorship W2015243129A5065020856 @default.
- W2015243129 hasAuthorship W2015243129A5081343739 @default.
- W2015243129 hasBestOaLocation W20152431292 @default.
- W2015243129 hasConcept C101762097 @default.
- W2015243129 hasConcept C104317684 @default.
- W2015243129 hasConcept C138885662 @default.
- W2015243129 hasConcept C150194340 @default.
- W2015243129 hasConcept C153911025 @default.
- W2015243129 hasConcept C179926584 @default.
- W2015243129 hasConcept C25281209 @default.
- W2015243129 hasConcept C2776449523 @default.
- W2015243129 hasConcept C41895202 @default.
- W2015243129 hasConcept C54355233 @default.
- W2015243129 hasConcept C64350747 @default.
- W2015243129 hasConcept C67705224 @default.
- W2015243129 hasConcept C70916841 @default.
- W2015243129 hasConcept C76346623 @default.
- W2015243129 hasConcept C86803240 @default.
- W2015243129 hasConceptScore W2015243129C101762097 @default.
- W2015243129 hasConceptScore W2015243129C104317684 @default.
- W2015243129 hasConceptScore W2015243129C138885662 @default.
- W2015243129 hasConceptScore W2015243129C150194340 @default.
- W2015243129 hasConceptScore W2015243129C153911025 @default.
- W2015243129 hasConceptScore W2015243129C179926584 @default.
- W2015243129 hasConceptScore W2015243129C25281209 @default.
- W2015243129 hasConceptScore W2015243129C2776449523 @default.
- W2015243129 hasConceptScore W2015243129C41895202 @default.
- W2015243129 hasConceptScore W2015243129C54355233 @default.
- W2015243129 hasConceptScore W2015243129C64350747 @default.
- W2015243129 hasConceptScore W2015243129C67705224 @default.
- W2015243129 hasConceptScore W2015243129C70916841 @default.
- W2015243129 hasConceptScore W2015243129C76346623 @default.
- W2015243129 hasConceptScore W2015243129C86803240 @default.
- W2015243129 hasIssue "12" @default.
- W2015243129 hasLocation W20152431291 @default.