FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015248839> ?p ?o ?g. }

• W2015248839 endingPage "2626" @default.
• W2015248839 startingPage "2615" @default.
• W2015248839 abstract "Recombinant adenoviruses are currently being used as vectors for gene delivery to a wide variety of cells and tissues. Although generally efficacious for gene transfer in vitro, improvement in the efficiency of vector delivery in vivo may aid several gene therapy applications. One major obstacle is the lack of high-affinity viral receptors on the surface of certain cells that are targets for gene therapy. In principle, incorporation of avid, cell-specific ligands into the virion could markedly improve vector entry into the desired tissues. We have developed a strategy for addressing this issue in the lung by biopanning differentiated, ciliated airway epithelial cells against a phage display library. The peptide with the most effective binding was coupled to the surface of an adenovirus using bifunctional polyethylene glycol (PEG) molecules. The chemically modified adenoviral vector was able to effect gene transfer to well-differentiated human airway epithelial cells by an alternative pathway dependent on the incorporated peptide. Coupling of PEG to the surface of the virus also served to partially protect the virus from neutralizing antibodies in vitro. These experiments will aid in the design of improved adenoviral vectors with the capacity for more specific and efficient delivery of therapeutic genes to desired target tissues. We have used a novel method for enhancing gene delivery to target cells by coupling a biologically selected peptide to the surface of an adenovirus with bifunctional PEG molecules. Modification of the viral capsid by the addition of a peptide with binding preference for differentiated ciliated airway epithelia allowed gene delivery to those cells by a novel entry pathway. Incorporation of the CFTR gene in a similarly modified vector resulted in correction of defective Cl- transport in well-differentiated epithelial cultures established from human cystic fibrosis (CF) donors. The presence of PEG molecules on the surface of the virus served, in addition, to reduce antibody neutralization. Modification of adenoviruses with PEG/peptide complexes can serve to partially overcome the barrier of inefficient gene transfer in some cell types and some of the adverse immunological responses associated with gene delivery by these vectors." @default.
• W2015248839 created "2016-06-24" @default.
• W2015248839 creator A5012917984 @default.
• W2015248839 creator A5028519710 @default.
• W2015248839 creator A5035364814 @default.
• W2015248839 creator A5038194611 @default.
• W2015248839 creator A5057269820 @default.
• W2015248839 creator A5089021019 @default.
• W2015248839 date "1999-11-10" @default.
• W2015248839 modified "2023-10-01" @default.
• W2015248839 title "Modification of an Adenoviral Vector with Biologically Selected Peptides: A Novel Strategy for Gene Delivery to Cells of Choice" @default.
• W2015248839 cites W1506470251 @default.
• W2015248839 cites W1547718427 @default.
• W2015248839 cites W1591942886 @default.
• W2015248839 cites W1831447929 @default.
• W2015248839 cites W1845317386 @default.
• W2015248839 cites W1971025152 @default.
• W2015248839 cites W1979273582 @default.
• W2015248839 cites W1984376606 @default.
• W2015248839 cites W1986769432 @default.
• W2015248839 cites W1988740847 @default.
• W2015248839 cites W1992677824 @default.
• W2015248839 cites W1998128870 @default.
• W2015248839 cites W1998162097 @default.
• W2015248839 cites W2009355062 @default.
• W2015248839 cites W2015617337 @default.
• W2015248839 cites W2017558860 @default.
• W2015248839 cites W2020301682 @default.
• W2015248839 cites W2039033043 @default.
• W2015248839 cites W2040016439 @default.
• W2015248839 cites W2051758487 @default.
• W2015248839 cites W2053944961 @default.
• W2015248839 cites W2065392104 @default.
• W2015248839 cites W2067048467 @default.
• W2015248839 cites W2078968390 @default.
• W2015248839 cites W2079791074 @default.
• W2015248839 cites W2087714421 @default.
• W2015248839 cites W2090270527 @default.
• W2015248839 cites W2090781939 @default.
• W2015248839 cites W2111871633 @default.
• W2015248839 cites W2116071714 @default.
• W2015248839 cites W2120138824 @default.
• W2015248839 cites W2129859001 @default.
• W2015248839 cites W2130451879 @default.
• W2015248839 cites W2133753843 @default.
• W2015248839 cites W2134430211 @default.
• W2015248839 cites W2147961945 @default.
• W2015248839 cites W2151534641 @default.
• W2015248839 cites W2164342253 @default.
• W2015248839 cites W2173640619 @default.
• W2015248839 doi "https://doi.org/10.1089/10430349950016654" @default.
• W2015248839 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10566889" @default.
• W2015248839 hasPublicationYear "1999" @default.
• W2015248839 type Work @default.
• W2015248839 sameAs 2015248839 @default.
• W2015248839 citedByCount "126" @default.
• W2015248839 countsByYear W20152488392012 @default.
• W2015248839 countsByYear W20152488392013 @default.
• W2015248839 countsByYear W20152488392014 @default.
• W2015248839 countsByYear W20152488392016 @default.
• W2015248839 countsByYear W20152488392017 @default.
• W2015248839 countsByYear W20152488392019 @default.
• W2015248839 countsByYear W20152488392020 @default.
• W2015248839 countsByYear W20152488392021 @default.
• W2015248839 countsByYear W20152488392023 @default.
• W2015248839 crossrefType "journal-article" @default.
• W2015248839 hasAuthorship W2015248839A5012917984 @default.
• W2015248839 hasAuthorship W2015248839A5028519710 @default.
• W2015248839 hasAuthorship W2015248839A5035364814 @default.
• W2015248839 hasAuthorship W2015248839A5038194611 @default.
• W2015248839 hasAuthorship W2015248839A5057269820 @default.
• W2015248839 hasAuthorship W2015248839A5089021019 @default.
• W2015248839 hasConcept C104317684 @default.
• W2015248839 hasConcept C104428545 @default.
• W2015248839 hasConcept C111599444 @default.
• W2015248839 hasConcept C135983454 @default.
• W2015248839 hasConcept C153911025 @default.
• W2015248839 hasConcept C159047783 @default.
• W2015248839 hasConcept C167625842 @default.
• W2015248839 hasConcept C202878990 @default.
• W2015248839 hasConcept C2522874641 @default.
• W2015248839 hasConcept C2776471321 @default.
• W2015248839 hasConcept C32470452 @default.
• W2015248839 hasConcept C40767141 @default.
• W2015248839 hasConcept C55493867 @default.
• W2015248839 hasConcept C86803240 @default.
• W2015248839 hasConcept C95444343 @default.
• W2015248839 hasConceptScore W2015248839C104317684 @default.
• W2015248839 hasConceptScore W2015248839C104428545 @default.
• W2015248839 hasConceptScore W2015248839C111599444 @default.
• W2015248839 hasConceptScore W2015248839C135983454 @default.
• W2015248839 hasConceptScore W2015248839C153911025 @default.
• W2015248839 hasConceptScore W2015248839C159047783 @default.
• W2015248839 hasConceptScore W2015248839C167625842 @default.
• W2015248839 hasConceptScore W2015248839C202878990 @default.
• W2015248839 hasConceptScore W2015248839C2522874641 @default.
• W2015248839 hasConceptScore W2015248839C2776471321 @default.
• W2015248839 hasConceptScore W2015248839C32470452 @default.

• next