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• W2015255280 abstract "• An LC–MS/MS method was developed and validated for the quantitation of the oncolytic drug tasquinimod in human urine. • The LLOQ of 0.100 nM (40 pg/mL) is 10-fold lower than previously reported for this compound in plasma. • Extraction with n -chlorobutane selectively removes endogenous urine compounds that interfere with the ionization process. • Urinary metabolites of tasquinimod can be back-converted to parent drug at 37 °C and at all relevant urine pH values (4.5–8.5). • The method was used to determine the urinary excretion of tasquinimod in healthy volunteers and renally impaired patients. Tasquinimod is an anti-tumor drug that is currently in clinical development for the treatment of solid cancers. After oral administration, tasquinimod and a number of its metabolites are excreted in the urine. The quantitative determination of tasquinimod in urine is challenging because of the required sensitivity (down to 0.1 nM or 40 pg/mL), the highly variable nature of this biological matrix and the presence of potentially unstable metabolites, which may convert back to the parent drug. In this article, an LC–MS/MS method is described for the determination of tasquinimod in human urine in the concentration range 0.1–200 nM. Liquid–liquid extraction with n -chlorobutane was used to extract tasquinimod from 100 μL human urine and to remove interfering endogenous urinary constituents. Reversed-phase liquid chromatography coupled to a triple quadrupole mass spectrometer equipped with an ESI source was used for quantification of tasquinimod in a 2.5-min run. A stable-isotope labeled internal standard was used for response normalization. The intra- and inter-day coefficients of variation (precision) as well as the bias (accuracy) of the method were below 7%. Although considerable conversion of conjugated tasquinimod metabolites back to parent drug was observed when incurred samples were stored at 37 °C for a prolonged time, tasquinimod as well as its metabolites were sufficiently stable under all relevant sampling, storage and analysis conditions. The method was successfully applied to determine the urinary excretion of tasquinimod in healthy volunteers and patients with renal impairment after a 0.5-mg oral dose." @default.
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• W2015255280 date "2014-06-01" @default.
• W2015255280 modified "2023-10-16" @default.
• W2015255280 title "Quantitative determination of the anti-tumor agent tasquinimod in human urine by liquid chromatography–tandem mass spectrometry" @default.
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• W2015255280 doi "https://doi.org/10.1016/j.jchromb.2014.05.007" @default.
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