Matches in SemOpenAlex for { <https://semopenalex.org/work/W2015280213> ?p ?o ?g. }
- W2015280213 endingPage "4182" @default.
- W2015280213 startingPage "4174" @default.
- W2015280213 abstract "A comprehensive totally aqueous phase synthesis of nickel-nitrilotriacetate (Ni-NTA) modified superparamagnetic Fe(3)O(4) nanoparticles is presented. The Fe(3)O(4)-NTA-Ni nanoparticles are able to perform efficient and specific purification of 6-His tagged proteins from crude cell lysates, as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The average binding capacity, as demonstrated by streptopain (M(W) 42 kDa), is 0.23 mg/mg (protein/Fe(3)O(4)-NTA-Ni). Considering the high affinity and specificity of the binding between hexahistidine motif and Ni-NTA, Ni-NTA modified nanoparticles could act as a module to carry 6-His tagged proteins on the particle surface with molecular orientation control, since only the 6-His domain could be attached. These modularly designed functional nanoparticles enhance cancer cell targeting, as supported by the in vitro receptor mediated targeting assay using RGD-4C-6-His fusion peptide. The nanoparticles show no significant hemolysis for human blood and could be investigated further for their in vivo functional imaging applications." @default.
- W2015280213 created "2016-06-24" @default.
- W2015280213 creator A5007401599 @default.
- W2015280213 creator A5008164688 @default.
- W2015280213 creator A5009138501 @default.
- W2015280213 creator A5025905021 @default.
- W2015280213 creator A5036098587 @default.
- W2015280213 creator A5038554977 @default.
- W2015280213 creator A5038962078 @default.
- W2015280213 creator A5049740282 @default.
- W2015280213 date "2006-08-01" @default.
- W2015280213 modified "2023-10-16" @default.
- W2015280213 title "Aqueous nickel-nitrilotriacetate modified Fe<sub>3</sub>O<sub>4</sub>–NH<sub>3</sub><sup>+</sup>nanoparticles for protein purification and cell targeting" @default.
- W2015280213 cites W1964670708 @default.
- W2015280213 cites W1968839949 @default.
- W2015280213 cites W1970998858 @default.
- W2015280213 cites W1983419175 @default.
- W2015280213 cites W2008741856 @default.
- W2015280213 cites W2011105478 @default.
- W2015280213 cites W2014811157 @default.
- W2015280213 cites W2016460723 @default.
- W2015280213 cites W2024294630 @default.
- W2015280213 cites W2041618472 @default.
- W2015280213 cites W2044487484 @default.
- W2015280213 cites W2046142060 @default.
- W2015280213 cites W2047252198 @default.
- W2015280213 cites W2053497072 @default.
- W2015280213 cites W2055322648 @default.
- W2015280213 cites W2060520167 @default.
- W2015280213 cites W2076541507 @default.
- W2015280213 cites W2077099192 @default.
- W2015280213 cites W2083972259 @default.
- W2015280213 cites W2091625916 @default.
- W2015280213 cites W2120269759 @default.
- W2015280213 cites W2132351075 @default.
- W2015280213 cites W2154890253 @default.
- W2015280213 cites W2159772188 @default.
- W2015280213 doi "https://doi.org/10.1088/0957-4484/17/16/030" @default.
- W2015280213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21727556" @default.
- W2015280213 hasPublicationYear "2006" @default.
- W2015280213 type Work @default.
- W2015280213 sameAs 2015280213 @default.
- W2015280213 citedByCount "30" @default.
- W2015280213 countsByYear W20152802132012 @default.
- W2015280213 countsByYear W20152802132013 @default.
- W2015280213 countsByYear W20152802132014 @default.
- W2015280213 countsByYear W20152802132015 @default.
- W2015280213 countsByYear W20152802132016 @default.
- W2015280213 countsByYear W20152802132017 @default.
- W2015280213 countsByYear W20152802132018 @default.
- W2015280213 countsByYear W20152802132019 @default.
- W2015280213 countsByYear W20152802132020 @default.
- W2015280213 countsByYear W20152802132022 @default.
- W2015280213 countsByYear W20152802132023 @default.
- W2015280213 crossrefType "journal-article" @default.
- W2015280213 hasAuthorship W2015280213A5007401599 @default.
- W2015280213 hasAuthorship W2015280213A5008164688 @default.
- W2015280213 hasAuthorship W2015280213A5009138501 @default.
- W2015280213 hasAuthorship W2015280213A5025905021 @default.
- W2015280213 hasAuthorship W2015280213A5036098587 @default.
- W2015280213 hasAuthorship W2015280213A5038554977 @default.
- W2015280213 hasAuthorship W2015280213A5038962078 @default.
- W2015280213 hasAuthorship W2015280213A5049740282 @default.
- W2015280213 hasConcept C101660505 @default.
- W2015280213 hasConcept C104317684 @default.
- W2015280213 hasConcept C116084860 @default.
- W2015280213 hasConcept C13965031 @default.
- W2015280213 hasConcept C155672457 @default.
- W2015280213 hasConcept C171250308 @default.
- W2015280213 hasConcept C178790620 @default.
- W2015280213 hasConcept C181199279 @default.
- W2015280213 hasConcept C184651966 @default.
- W2015280213 hasConcept C185592680 @default.
- W2015280213 hasConcept C192562407 @default.
- W2015280213 hasConcept C2776415932 @default.
- W2015280213 hasConcept C55493867 @default.
- W2015280213 hasConceptScore W2015280213C101660505 @default.
- W2015280213 hasConceptScore W2015280213C104317684 @default.
- W2015280213 hasConceptScore W2015280213C116084860 @default.
- W2015280213 hasConceptScore W2015280213C13965031 @default.
- W2015280213 hasConceptScore W2015280213C155672457 @default.
- W2015280213 hasConceptScore W2015280213C171250308 @default.
- W2015280213 hasConceptScore W2015280213C178790620 @default.
- W2015280213 hasConceptScore W2015280213C181199279 @default.
- W2015280213 hasConceptScore W2015280213C184651966 @default.
- W2015280213 hasConceptScore W2015280213C185592680 @default.
- W2015280213 hasConceptScore W2015280213C192562407 @default.
- W2015280213 hasConceptScore W2015280213C2776415932 @default.
- W2015280213 hasConceptScore W2015280213C55493867 @default.
- W2015280213 hasIssue "16" @default.
- W2015280213 hasLocation W20152802131 @default.
- W2015280213 hasLocation W20152802132 @default.
- W2015280213 hasOpenAccess W2015280213 @default.
- W2015280213 hasPrimaryLocation W20152802131 @default.
- W2015280213 hasRelatedWork W1981184620 @default.
- W2015280213 hasRelatedWork W2006420327 @default.
- W2015280213 hasRelatedWork W2015967489 @default.
- W2015280213 hasRelatedWork W2030993022 @default.